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Literature summary extracted from

  • Palczewski, K.; Kahn, N.; Hargrave, P.A.
    Nucleoside inhibitors of rhodopsin kinase (1990), Biochemistry, 29, 6276-6282.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 1-(6-Amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-furanuronamide good inhibitor Bos taurus
2.7.11.14 2-chloroadenosine
-
 Bos taurus
2.7.11.14 5'-(N-ethylcarbamoyl)adenosine
-
 Bos taurus
2.7.11.14 5'-AMP
-
 Bos taurus
2.7.11.14 5,6-Dichloro-1-(beta-ribofuranosyl)-benzimidazole
-
 Bos taurus
2.7.11.14 8,2'-Anhydro-8-mercapto-9-(beta-D-arabinofuranosyl)adenine weak Bos taurus
2.7.11.14 8,3'-Anhydro-8-oxy-9-(beta-D-xylofuranosyl)adenine good inhibitor Bos taurus
2.7.11.14 8,5'-Anhydro-8-oxy-9-(beta-D-ribofuranosyl)adenine
-
 Bos taurus
2.7.11.14 8-Bromoadenosine good inhibitor Bos taurus
2.7.11.14 adenine
-
 Bos taurus
2.7.11.14 adenosine
-
 Bos taurus
2.7.11.14 ADP
-
 Bos taurus
2.7.11.14 ADPbetaS
-
 Bos taurus
2.7.11.14 AMPS
-
 Bos taurus
2.7.11.14 ATPalphaS S-isomer Bos taurus
2.7.11.14 diphosphate
-
 Bos taurus
2.7.11.14 Formycin monophosphate
-
 Bos taurus
2.7.11.14 isoquinoline derivative
-
 Bos taurus
2.7.11.14 additional information inhibition studies with adenosine analogues, not inhibited by ribose 5-phosphate, ethenoadenosine, 8-bromo-AMP, 8,2'-anhydro-8-oxy-9-(beta-D-arabinofuranosyl)adenine Bos taurus
2.7.11.14 nucleoside analogue overview Bos taurus
2.7.11.14 purine nucleotide overview Bos taurus
2.7.11.14 pyrrolopyrimidine derivative preferentially in anti-configuration, strong Bos taurus
2.7.11.14 Sangivamycin strong, in vivo and in vitro Bos taurus
2.7.11.14 Toyocamycin strong Bos taurus
2.7.11.14 Triphosphate
-
 Bos taurus
2.7.11.14 tubercidin 5'-phosphate good inhibitor Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.14 0.0016 0.002 ATP pH 7.5, 30°C, cosubstrate rhodopsin, membrane-bound Bos taurus
2.7.11.14 0.027
-
 ATPgammaS pH 7.5, 30°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.14 ATP + rhodopsin = ADP + phosphorhodopsin mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.5 0.85 pH 7.5, 30°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin specificity of ATP-binding site Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: ATP Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin highly specific for photobleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin 5-6 mol phosphate/mol rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATPgammaS + rhodopsin ATPgammaS is a good substrate, 2-3 mol phosphate/mol rhodopsin Bos taurus ?
-
 ?
2.7.11.14 GTP + rhodopsin can replace ATP to a lesser extent Bos taurus GDP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.14 additional information
-
 additional information
-
 Bos taurus
2.7.11.14 0.00018
-
 Sangivamycin pH 7.5, 30°C Bos taurus
2.7.11.14 0.0036
-
 tubercidin 5'-phosphate pH 7.5, 30°C Bos taurus
2.7.11.14 0.004
-
 adenosine pH 7.5, 30°C Bos taurus
2.7.11.14 0.004
-
 5,6-Dichloro-1-(beta-ribofuranosyl)-benzimidazole pH 7.5, 30°C Bos taurus
2.7.11.14 0.005
-
 5'-AMP pH 7.5, 30°C Bos taurus
2.7.11.14 0.0087
-
 adenine pH 7.5, 30°C Bos taurus
2.7.11.14 0.01
-
 8,3'-Anhydro-8-oxy-9-(beta-D-xylofuranosyl)adenine pH 7.5, 30°C Bos taurus
2.7.11.14 0.012
-
 ADP pH 7.5, 30°C Bos taurus
2.7.11.14 0.014
-
 Triphosphate pH 7.5, 30°C Bos taurus
2.7.11.14 0.016
-
 1-(6-Amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-furanuronamide pH 7.5, 30°C Bos taurus
2.7.11.14 0.02
-
 8-Bromoadenosine pH 7.5, 30°C Bos taurus
2.7.11.14 0.022
-
 ADPbetaS pH 7.5, 30°C Bos taurus
2.7.11.14 0.022
-
 AMPS pH 7.5, 30°C Bos taurus
2.7.11.14 0.024
-
 diphosphate pH 7.5, 30°C Bos taurus
2.7.11.14 0.027
-
 8,5'-Anhydro-8-oxy-9-(beta-D-ribofuranosyl)adenine pH 7.5, 30°C Bos taurus
2.7.11.14 0.035
-
 ATPalphaS pH 7.5, 30°C, S-isomer Bos taurus
2.7.11.14 0.036
-
 Formycin monophosphate pH 7.5, 30°C Bos taurus
2.7.11.14 0.52
-
 8,2'-Anhydro-8-mercapto-9-(beta-D-arabinofuranosyl)adenine pH 7.5, 30°C Bos taurus