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Literature summary extracted from

  • Sellers, J.R.; Harvey, E.V.
    Purification of myosin light chain kinase from Limulus muscle (1984), Biochemistry, 23, 5821-5826.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 autophosphorylation no activation Limulus sp.
2.7.11.18 phosphorylation no activation Limulus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.0156
-
 Limulus myosin light chain 25°C, pH 7.5 Limulus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+
-
 Limulus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 37000
-
 PAGE, 2 forms of myosin light chain kinase Limulus sp.
2.7.11.18 39000
-
 PAGE, 2 forms of myosin light chain kinase Limulus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Limulus sp.
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Limulus sp.
-
 horseshoe crab
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18
-
 Limulus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 telson muscle Limulus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are actin and tropomyosin Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone 2-A Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone 2 b Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone H1 Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain 1 mol phosphate per mol light chain in skeletal muscle Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Limulus sp. ADP + myosin light chain phosphate
-
 ?
2.7.11.18 additional information no intramolecular autophosphorylation in the absence of acceptor substrate Limulus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 monomer 1 * 37000, SDS-PAGE 2 forms of myosin light chain kinase Limulus sp.
2.7.11.18 monomer 1 * 39000, SDS-PAGE, 2 forms of myosin light chain kinase Limulus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Limulus sp.