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Literature summary extracted from

  • Adelstein, R.S.; Klee, C.B.
    Purification and characterization of smooth muscle myosin light chain kinase (1981), J. Biol. Chem., 256, 7501-7509.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.18 EGTA prevents Ca2+-dependent proteolysis during initial purification Meleagris gallopavo
2.7.11.18 MgCl2 is critical for kinase extraction from myofibrils Meleagris gallopavo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.005
-
 myosin light chain 24°C, pH 7.3 Meleagris gallopavo
2.7.11.18 0.05
-
 ATP 24°C, pH 7.3 Meleagris gallopavo

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.18 myofibril associated Meleagris gallopavo 30016
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+
-
 Meleagris gallopavo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 additional information
-
-
 Meleagris gallopavo
2.7.11.18 124000
-
 sedimentation equilibrium centrifugation Meleagris gallopavo
2.7.11.18 130000
-
 gel filtration Meleagris gallopavo

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Meleagris gallopavo
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Meleagris gallopavo
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 affinity chromatography on calmodulin-Sepharose Meleagris gallopavo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 gizzard
-
 Meleagris gallopavo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 6
-
-
 Meleagris gallopavo

Storage Stability

EC Number Storage Stability Organism
2.7.11.18 -30°C, 30% loss of activity within 3 weeks Meleagris gallopavo
2.7.11.18 -70°C, at least 6 months Meleagris gallopavo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle myosin Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of skeletal muscle Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of cardiac muscle Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone 2-A Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase kinase Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone V-S Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Meleagris gallopavo ADP + myosin light chain phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 monomer 1 * 130000, SDS-PAGE Meleagris gallopavo

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 24
-
 assay at Meleagris gallopavo

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Meleagris gallopavo