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Literature summary extracted from

  • Walsh, M.P.; Vallet, B.; Autric, F.; Demaille, J.G.
    Purification and characterization of bovine cardiac calmodulin-dependent myosin light chain kinase (1979), J. Biol. Chem., 254, 12136-12144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 additional information no activation by mild proteolysis Bos taurus
2.7.11.18 phosphorylation activation, smooth muscle enzyme, not bovine cardiac enzyme Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 Increasing ionic strength
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.175
-
 ATP 30°C, pH 8 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.18 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+
-
 Bos taurus
2.7.11.18 Mg2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 85000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Bos taurus
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 cardiac muscle myocardium Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are myosin heavy chain and phosvitin Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of skeletal muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of cardiac muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone 2-A Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain highly specific for regulatory or P-light-chain Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain highly specific for 18 kDa light chain Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase kinase Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain specific for 20 kDa light chain Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 additional information the enzyme possesses ATPase activity Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 monomer 1 * 85000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.18 8.1
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.18 6.8 8.8 about half-maximal activity at pH 6.8 and about 75% of maximal activity at pH 8.8 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.11.18 5
-
 30-60 min, about 50% loss of activity Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Bos taurus
2.7.11.18 Calmodulin only active as ternary complex of calmodulin, Ca2+ and kinase: activation is initiated by binding of Ca2+ to calmodulin Bos taurus