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Literature summary extracted from

  • Nagamoto, H.; Yagi, K.
    Properties of myosin light chain kinase prepared from rabbit skeletal muscle by an improved method (1984), J. Biochem., 95, 1119-1130.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.18 unstable upon lyophilization Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 KCl
-
 Oryctolagus cuniculus
2.7.11.18 NaCl
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.019
-
 myosin light chain 25°C, pH 7.6, bound to myosin Oryctolagus cuniculus
2.7.11.18 0.05 0.063 isolated myosin light chain 25°C, pH 7.6 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 additional information
-
 amino acid composition of rabbit Oryctolagus cuniculus
2.7.11.18 94000
-
 1 * 94000, SDS-PAGE Oryctolagus cuniculus
2.7.11.18 103000
-
 sedimentation equilibrium method Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Oryctolagus cuniculus
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 affinity chromatography on calmodulin-Sepharose Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 24
-
 skeletal muscle Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of skeletal muscle Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 monomer 1 * 94000, SDS-PAGE Oryctolagus cuniculus
2.7.11.18 More gel electrophoresis in various buffers gives different molecular weights Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 25
-
 assay at Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.18 16
-
 myosin light chain 25°C, pH 7.6, isolated Oryctolagus cuniculus
2.7.11.18 19
-
 myosin light chain 25°C, pH 7.6, bound to myosin Oryctolagus cuniculus
2.7.11.18 88
-
 myosin light chain 25°C, pH 7.6, isolated, freshly prepared Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.18 6.3 9.2 about half-maximal activity at pH 6.3 and 9.2 Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.11.18 5
-
 rapid inactivation below Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Oryctolagus cuniculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.18 Oryctolagus cuniculus isoelectric focusing, pH 3-10, 4% polyacrylamide gel or flat bed isoelectric focusing with Sephadex G-200
-
 5