BRENDA - Enzyme Database

Locations of the regulatory sites for isocitrate dehydrogenase kinase/phosphatase

Miller, S.P.; Chen, R.; Karschnia, E.J.; Romfo, C.; Dean, A.; LaPorte, D.C.; J. Biol. Chem. 275, 833-839 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.5
3-phosphoglycerate
-
Escherichia coli
2.7.11.5
AMP
-
Escherichia coli
2.7.11.5
DL-isocitrate
inhibits only kinase activity
Escherichia coli
2.7.11.5
NADPH
inhibits both IDH kinase and IDH phosphatase
Escherichia coli
2.7.11.5
pyruvate
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.11.5
0.0009
-
[isocitrate dehydrogenase (NADP+)]N15L
pH 7.5, 37C
Escherichia coli
2.7.11.5
0.0017
-
[isocitrate dehydrogenase (NADP+)]
pH 7.5, 37C
Escherichia coli
2.7.11.5
0.02
-
[isocitrate dehydrogenase (NADP+)]IS
pH 7.5, 37C
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
Escherichia coli
-
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
Escherichia coli
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.11.5
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
-
640609
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
640609
Escherichia coli
r
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]IS
-
640609
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)]IS phosphate
-
-
-
?
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]N115L
-
640609
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)]N115L phosphate
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.11.5
0.008
-
AMP
pH 7.5, 37C
Escherichia coli
2.7.11.5
0.011
-
isocitrate
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
0.015
-
isocitrate
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
0.016
-
isocitrate
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
0.02
-
AMP
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
0.058
-
NADPH
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
0.073
-
NADPH
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
0.082
-
NADPH
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
0.17
-
AMP
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
0.2
-
pyruvate
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
1
-
3-phosphoglycerate
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
1
-
pyruvate
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
4
-
3-phosphoglycerate
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
4
-
pyruvate
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
20
-
3-phosphoglycerate
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.5
3-phosphoglycerate
-
Escherichia coli
2.7.11.5
AMP
-
Escherichia coli
2.7.11.5
DL-isocitrate
inhibits only kinase activity
Escherichia coli
2.7.11.5
NADPH
inhibits both IDH kinase and IDH phosphatase
Escherichia coli
2.7.11.5
pyruvate
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.11.5
0.008
-
AMP
pH 7.5, 37C
Escherichia coli
2.7.11.5
0.011
-
isocitrate
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
0.015
-
isocitrate
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
0.016
-
isocitrate
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
0.02
-
AMP
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
0.058
-
NADPH
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
0.073
-
NADPH
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
0.082
-
NADPH
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
0.17
-
AMP
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
0.2
-
pyruvate
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
1
-
3-phosphoglycerate
pH 7.5, 37C, wild-type, kinase activity
Escherichia coli
2.7.11.5
1
-
pyruvate
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
4
-
3-phosphoglycerate
pH 7.5, 37C, mutant AceK4, kinase activity
Escherichia coli
2.7.11.5
4
-
pyruvate
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
2.7.11.5
20
-
3-phosphoglycerate
pH 7.5, 37C, mutant AceK3, kinase activity
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.11.5
0.0009
-
[isocitrate dehydrogenase (NADP+)]N15L
pH 7.5, 37C
Escherichia coli
2.7.11.5
0.0017
-
[isocitrate dehydrogenase (NADP+)]
pH 7.5, 37C
Escherichia coli
2.7.11.5
0.02
-
[isocitrate dehydrogenase (NADP+)]IS
pH 7.5, 37C
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
Escherichia coli
-
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
Escherichia coli
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
-
640609
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
640609
Escherichia coli
r
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]IS
-
640609
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)]IS phosphate
-
-
-
?
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]N115L
-
640609
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)]N115L phosphate
-
-
-
?