BRENDA - Enzyme Database

The regulatory properties of isocitrate dehydrogenase kinase and isocitrate dehydrogenase phosphatase from Escherichia coli ML308 and the roles of these activities in the control of isocitrate dehydrogenase

Nimmo, G.A.; Nimmo, H.G.; Eur. J. Biochem. 141, 409-414 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.5
2-oxoglutarate
inhibits kinase activity
Escherichia coli
2.7.11.5
ADP
kinase hyperbolically inhibited
Escherichia coli
2.7.11.5
AMP
kinase hyperbolically inhibited
Escherichia coli
2.7.11.5
DL-isocitrate
inhibits only kinase activity
Escherichia coli
2.7.11.5
glyoxylate
-
Escherichia coli
2.7.11.5
NADPH
-
Escherichia coli
2.7.11.5
oxaloacetate
inhibits kinase activity
Escherichia coli
2.7.11.5
phosphoenolpyruvate
kinase hyperbolically inhibited
Escherichia coli
2.7.11.5
pyruvate
inhibits kinase activity
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.11.5
0.00035
-
[isocitrate dehydrogenase (NADP+)]
pH 7.3, 37C, kinase activity
Escherichia coli
2.7.11.5
0.088
-
ATP
pH 7.3, 37C
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.11.5
Mg2+
absolute requirement, isocitrate dehydrogenase phosphatase responds hyperbolically to Mg2+ ions
Escherichia coli
2.7.11.5
additional information
Mn2+ or Ca2+ cannot replace Mg2+
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
Escherichia coli
phosphorylation of isocitrate dehydrogenase during growth on acetate is to render this enzyme rate-limiting in the citric acid cycle, this should cause an increase in the level of isocitrate and divert the flux of carbon through the glyoxylate bypass
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
Escherichia coli
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.11.5
Escherichia coli
-
ML308, ATCC 15224
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
-
640602
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
640602
Escherichia coli
r
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
phosphorylation of isocitrate dehydrogenase during growth on acetate is to render this enzyme rate-limiting in the citric acid cycle, this should cause an increase in the level of isocitrate and divert the flux of carbon through the glyoxylate bypass
640602
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
640602
Escherichia coli
r
2.7.11.5
additional information
uses only ATP, no other nucleoside triphospates as only very poor phosphate donors for the kinase activity, GTP and UTP can activate the phosphatase activity to some extent
640602
Escherichia coli
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.11.5
8
8.5
kinase activity
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.11.5
ADP
isocitrate dehydrogenase phosphatase requires a nucleotide for activity
Escherichia coli
2.7.11.5
ATP
isocitrate dehydrogenase phosphatase requires a nucleotide for activity
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.11.5
0.023
-
DL-isocitrate
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.042
-
NADPH
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.056
-
AMP
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.45
-
ADP
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.55
-
phosphoenolpyruvate
pH 7.3, 37C
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.11.5
ADP
isocitrate dehydrogenase phosphatase requires a nucleotide for activity
Escherichia coli
2.7.11.5
ATP
isocitrate dehydrogenase phosphatase requires a nucleotide for activity
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.11.5
2-oxoglutarate
inhibits kinase activity
Escherichia coli
2.7.11.5
ADP
kinase hyperbolically inhibited
Escherichia coli
2.7.11.5
AMP
kinase hyperbolically inhibited
Escherichia coli
2.7.11.5
DL-isocitrate
inhibits only kinase activity
Escherichia coli
2.7.11.5
glyoxylate
-
Escherichia coli
2.7.11.5
NADPH
-
Escherichia coli
2.7.11.5
oxaloacetate
inhibits kinase activity
Escherichia coli
2.7.11.5
phosphoenolpyruvate
kinase hyperbolically inhibited
Escherichia coli
2.7.11.5
pyruvate
inhibits kinase activity
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.11.5
0.023
-
DL-isocitrate
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.042
-
NADPH
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.056
-
AMP
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.45
-
ADP
pH 7.3, 37C
Escherichia coli
2.7.11.5
0.55
-
phosphoenolpyruvate
pH 7.3, 37C
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.11.5
0.00035
-
[isocitrate dehydrogenase (NADP+)]
pH 7.3, 37C, kinase activity
Escherichia coli
2.7.11.5
0.088
-
ATP
pH 7.3, 37C
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.11.5
Mg2+
absolute requirement, isocitrate dehydrogenase phosphatase responds hyperbolically to Mg2+ ions
Escherichia coli
2.7.11.5
additional information
Mn2+ or Ca2+ cannot replace Mg2+
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
Escherichia coli
phosphorylation of isocitrate dehydrogenase during growth on acetate is to render this enzyme rate-limiting in the citric acid cycle, this should cause an increase in the level of isocitrate and divert the flux of carbon through the glyoxylate bypass
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
Escherichia coli
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
-
640602
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
640602
Escherichia coli
r
2.7.11.5
ATP + [isocitrate dehydrogenase (NADP+)]
phosphorylation of isocitrate dehydrogenase during growth on acetate is to render this enzyme rate-limiting in the citric acid cycle, this should cause an increase in the level of isocitrate and divert the flux of carbon through the glyoxylate bypass
640602
Escherichia coli
ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
640602
Escherichia coli
r
2.7.11.5
additional information
uses only ATP, no other nucleoside triphospates as only very poor phosphate donors for the kinase activity, GTP and UTP can activate the phosphatase activity to some extent
640602
Escherichia coli
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.11.5
8
8.5
kinase activity
Escherichia coli