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Literature summary extracted from

  • Ikeda, S.; Ma, G.T.; Ives, D.H.
    Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors (1994), Biochemistry, 33, 5328-5334.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.76 dATP stabilizes Lactobacillus acidophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.76 dATP kinetic, mechanism, protects corresponding active site against trypsin inactivation Lactobacillus acidophilus
2.7.1.76 dCTP kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP Lactobacillus acidophilus
2.7.1.76 dGTP kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP Lactobacillus acidophilus
2.7.1.76 Trypsin substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites Lactobacillus acidophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+
-
 Lactobacillus acidophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 27200
-
 1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure Lactobacillus acidophilus
2.7.1.74 29000
-
 1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure Lactobacillus acidophilus
2.7.1.76 27200
-
 1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE Lactobacillus acidophilus
2.7.1.76 29000
-
 1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE Lactobacillus acidophilus
2.7.1.113 27200
-
 2 * 27200, heterodimeric dAK/dGK complex, SDS-PAGE Lactobacillus acidophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Lactobacillus acidophilus
-
-
-
2.7.1.76 Lactobacillus acidophilus
-
 strain R-26
-
2.7.1.76 Lactobacillus acidophilus ATCC 700396
-
 strain R-26
-
2.7.1.113 Lactobacillus acidophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 affinity chromatography Lactobacillus acidophilus
2.7.1.76 affinity chromatography Lactobacillus acidophilus

Storage Stability

EC Number Storage Stability Organism
2.7.1.76 dATP stabilizes during storage Lactobacillus acidophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 NTP + deoxyadenosine
-
 Lactobacillus acidophilus NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxyadenosine
-
 Lactobacillus acidophilus ATCC 700396 NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Lactobacillus acidophilus NDP + dCMP
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Lactobacillus acidophilus ATCC 700396 NDP + dCMP
-
 ?
2.7.1.76 ATP + deoxyadenosine enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase Lactobacillus acidophilus ADP + dAMP
-
 ?
2.7.1.76 ATP + deoxyadenosine enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase Lactobacillus acidophilus ATCC 700396 ADP + dAMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 dimer 1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure Lactobacillus acidophilus
2.7.1.76 dimer 1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE Lactobacillus acidophilus
2.7.1.76 More both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits Lactobacillus acidophilus
2.7.1.113 dimer 2 * 27200, heterodimeric dAK/dGK complex, SDS-PAGE Lactobacillus acidophilus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.76 dCyd kinase/dAdo kinase I
-
 Lactobacillus acidophilus
2.7.1.76 dGuo kinase/dAdo kinase II
-
 Lactobacillus acidophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.76 20
-
 assay at Lactobacillus acidophilus