show Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors
Ikeda, S.; Ma, G.T.; Ives, D.H.; Biochemistry 33, 5328-5334 (1994)
Data extracted from this reference:
General Stability
EC Number
General Stability
Organism
2.7.1.76
dATP stabilizes
Lactobacillus acidophilus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
dATP
kinetic, mechanism, protects corresponding active site against trypsin inactivation
Lactobacillus acidophilus
2.7.1.76
dCTP
kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
Lactobacillus acidophilus
2.7.1.76
dGTP
kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
Lactobacillus acidophilus
2.7.1.76
Trypsin
substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites
Lactobacillus acidophilus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Lactobacillus acidophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
27200
-
1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure
Lactobacillus acidophilus
2.7.1.74
29000
-
1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure
Lactobacillus acidophilus
2.7.1.76
27200
-
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
Lactobacillus acidophilus
2.7.1.76
29000
-
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
Lactobacillus acidophilus
2.7.1.113
27200
-
2 * 27200, heterodimeric dAK/dGK complex, SDS-PAGE
Lactobacillus acidophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Lactobacillus acidophilus
-
-
-
2.7.1.76
Lactobacillus acidophilus
-
strain R-26
-
2.7.1.76
Lactobacillus acidophilus ATCC 700396
-
strain R-26
-
2.7.1.113
Lactobacillus acidophilus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography
Lactobacillus acidophilus
2.7.1.76
affinity chromatography
Lactobacillus acidophilus
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.76
dATP stabilizes during storage
Lactobacillus acidophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
NTP + deoxyadenosine
-
640566
Lactobacillus acidophilus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
640566
Lactobacillus acidophilus ATCC 700396
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
640566
Lactobacillus acidophilus
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
640566
Lactobacillus acidophilus ATCC 700396
NDP + dCMP
-
-
-
?
2.7.1.76
ATP + deoxyadenosine
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
640566
Lactobacillus acidophilus
ADP + dAMP
-
640566
Lactobacillus acidophilus
?
2.7.1.76
ATP + deoxyadenosine
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
640566
Lactobacillus acidophilus ATCC 700396
ADP + dAMP
-
640566
Lactobacillus acidophilus ATCC 700396
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure
Lactobacillus acidophilus
2.7.1.76
dimer
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
Lactobacillus acidophilus
2.7.1.76
More
both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits
Lactobacillus acidophilus
2.7.1.113
dimer
2 * 27200, heterodimeric dAK/dGK complex, SDS-PAGE
Lactobacillus acidophilus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
20
-
assay at
Lactobacillus acidophilus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.76
dATP stabilizes
Lactobacillus acidophilus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
dATP
kinetic, mechanism, protects corresponding active site against trypsin inactivation
Lactobacillus acidophilus
2.7.1.76
dCTP
kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
Lactobacillus acidophilus
2.7.1.76
dGTP
kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
Lactobacillus acidophilus
2.7.1.76
Trypsin
substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites
Lactobacillus acidophilus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Lactobacillus acidophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.74
27200
-
1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure
Lactobacillus acidophilus
2.7.1.74
29000
-
1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure
Lactobacillus acidophilus
2.7.1.76
27200
-
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
Lactobacillus acidophilus
2.7.1.76
29000
-
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
Lactobacillus acidophilus
2.7.1.113
27200
-
2 * 27200, heterodimeric dAK/dGK complex, SDS-PAGE
Lactobacillus acidophilus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
affinity chromatography
Lactobacillus acidophilus
2.7.1.76
affinity chromatography
Lactobacillus acidophilus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.76
dATP stabilizes during storage
Lactobacillus acidophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
NTP + deoxyadenosine
-
640566
Lactobacillus acidophilus
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxyadenosine
-
640566
Lactobacillus acidophilus ATCC 700396
NDP + dAMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
640566
Lactobacillus acidophilus
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
640566
Lactobacillus acidophilus ATCC 700396
NDP + dCMP
-
-
-
?
2.7.1.76
ATP + deoxyadenosine
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
640566
Lactobacillus acidophilus
ADP + dAMP
-
640566
Lactobacillus acidophilus
?
2.7.1.76
ATP + deoxyadenosine
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
640566
Lactobacillus acidophilus ATCC 700396
ADP + dAMP
-
640566
Lactobacillus acidophilus ATCC 700396
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
dimer
1 * 27200 + 1 * 29000, SDS-PAGE, heterodimers in quarternary structure
Lactobacillus acidophilus
2.7.1.76
dimer
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
Lactobacillus acidophilus
2.7.1.76
More
both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits
Lactobacillus acidophilus
2.7.1.113
dimer
2 * 27200, heterodimeric dAK/dGK complex, SDS-PAGE
Lactobacillus acidophilus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
20
-
assay at
Lactobacillus acidophilus