BRENDA - Enzyme Database show

Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor

Andersen, R.B.; Neuhard, J.; J. Biol. Chem. 276, 5518-5524 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.76
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
2.7.1.113
expression in Escherichia coli; gene yaaG of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
General Stability
EC Number
General Stability
Organism
2.7.1.113
bovine serum albumin stabilizes the enzyme in solution
Bacillus subtilis
2.7.1.113
highly unstable at 0°C in diluted form, no loss of activity in the presence of bovine serum albumin
Bacillus subtilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.113
dGMP
noncompetitive versus UTP and 2'-deoxyguanosine
Bacillus subtilis
2.7.1.113
additional information
no substrate inhibition by CTP, ATP, and GTP
Bacillus subtilis
2.7.1.113
UDP
competitive versus UTP, noncompetitive versus 2'-deoxyguanosine
Bacillus subtilis
2.7.1.113
UTP
substrate inhibition, overview
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.0006
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP; pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
2.7.1.113
0.0017
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
2.7.1.113
0.006
-
UTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
0.0065
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP; pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
2.7.1.113
0.01
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
2.7.1.113
0.0104
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
2.7.1.113
0.017
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
2.7.1.113
0.035
-
CTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
0.036
-
ATP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
0.046
-
GTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Mg2+
required for activity
Bacillus subtilis
2.7.1.113
Mg2+
MgNTP2- is most probably the true phosphate donor; required for activity; required for activity, no inhibition by excess Mg2+, at 5 mM
Bacillus subtilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.113
24147
-
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
2.7.1.113
49000
-
recombinant deoxyguanosine kinase, gel filtration; recombinant enzyme, gel filtration
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Bacillus subtilis
-
gene yaaF, yaaF encodes an enzyme with specificity for deoxyadenosine and deoxycytidine
-
2.7.1.113
Bacillus subtilis
-
; gene yaaG
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.113
recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose; recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity
Bacillus subtilis
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.113
ATP + deoxyguanosine = ADP + dGMP
sequential Bi Bi mechanism
Bacillus subtilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
2.7.1.113
0.54
-
enzyme in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyguanosine
Bacillus subtilis
2.7.1.113
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
2.7.1.113
8.1
-
purified recombinant enzyme, substrate 2'-deoxyguanosine; recombinant deoxyguanosine kinase
Bacillus subtilis
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.113
-20°C, more than 1 mg/ml protein, 50% glycerol, 1 year, no loss of activity
Bacillus subtilis
2.7.1.113
-20°C, purified recombinant enzyme at 1 mg/ml, Tris-HCl, pH 7.8, 50% v/v glycerol, at least 1 year without loss of activity
Bacillus subtilis
2.7.1.113
0°C, the diluted enzyme is highly unstable, but in presence of 1 mg/ml bovine serum albumin, the diluted enzyme is stable for months
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
GTP + 2'-deoxyadenosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.76
GTP + 2'-deoxycytidine
-
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
additional information
substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine
640561
Bacillus subtilis
?
-
-
-
-
2.7.1.113
ATP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + deoxyadenosine
3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dAMP
-
-
-
?
2.7.1.113
ATP + deoxycytidine
recombinant deoxyguanosine kinase, 3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dCMP
-
-
-
?
2.7.1.113
ATP + deoxyinosine
63% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dIMP
-
640561
Bacillus subtilis
?
2.7.1.113
ATP + guanosine
recombinant deoxyguanosine kinase, 7% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + GMP
-
640561
Bacillus subtilis
?
2.7.1.113
CTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
CDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxyadenosine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxycytidine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxyinosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + guanosine
very low activity
640561
Bacillus subtilis
GDP + guanosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
substrate specificity, the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors, and shows poor activity with thymidine, inosine, adenosine, 2,3-dideoxyguanosine, 2,3-dideoxyinosine, 2,3-dideoxyadenosine, and hypoxanthine arabinoside as acceptor substrates
640561
Bacillus subtilis
?
-
-
-
-
2.7.1.113
UTP + 2'-deoxyguanosine
preferred substrates
640561
Bacillus subtilis
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.113
dimer
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Bacillus subtilis
2.7.1.113
37
-
assay at
Bacillus subtilis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.113
1.41
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
2.7.1.113
1.41
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
2.7.1.113
1.41
-
UTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
1.53
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
2.7.1.113
1.53
-
ATP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
1.53
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP
Bacillus subtilis
2.7.1.113
1.69
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
2.7.1.113
1.69
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
2.7.1.113
1.69
-
GTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
2.25
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
2.7.1.113
2.25
-
CTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
2.25
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.76
8.5
-
assay at
Bacillus subtilis
2.7.1.113
9
-
; broad optimum
Bacillus subtilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.113
5.6
9
more than 80% of maximal activity at pH 7.5 and pH 11.5, respectively, 60% of maximal activity at pH 6.0, complete loss of activity below pH 5.6
Bacillus subtilis
2.7.1.113
6
11.5
over 80% of maximal activity at pH 7.5 and pH 11.5, about 60% of maximal activity at pH 6.0, enzyme is completely inactive below pH 5.6
Bacillus subtilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
GTP
-
Bacillus subtilis
2.7.1.113
ATP
-
Bacillus subtilis
2.7.1.113
CTP
-
Bacillus subtilis
2.7.1.113
GTP
-
Bacillus subtilis
2.7.1.113
additional information
the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors
Bacillus subtilis
2.7.1.113
UTP
-
Bacillus subtilis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.113
additional information
-
additional information
product inhibition of the enzyme, overview
Bacillus subtilis
2.7.1.113
0.063
-
UDP
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
2.7.1.113
expression in Escherichia coli; gene yaaG of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
GTP
-
Bacillus subtilis
2.7.1.113
ATP
-
Bacillus subtilis
2.7.1.113
CTP
-
Bacillus subtilis
2.7.1.113
GTP
-
Bacillus subtilis
2.7.1.113
additional information
the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors
Bacillus subtilis
2.7.1.113
UTP
-
Bacillus subtilis
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.113
bovine serum albumin stabilizes the enzyme in solution
Bacillus subtilis
2.7.1.113
highly unstable at 0°C in diluted form, no loss of activity in the presence of bovine serum albumin
Bacillus subtilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.113
dGMP
noncompetitive versus UTP and 2'-deoxyguanosine
Bacillus subtilis
2.7.1.113
additional information
no substrate inhibition by CTP, ATP, and GTP
Bacillus subtilis
2.7.1.113
UDP
competitive versus UTP, noncompetitive versus 2'-deoxyguanosine
Bacillus subtilis
2.7.1.113
UTP
substrate inhibition, overview
Bacillus subtilis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.113
additional information
-
additional information
product inhibition of the enzyme, overview
Bacillus subtilis
2.7.1.113
0.063
-
UDP
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.0006
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP; pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
2.7.1.113
0.0017
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
2.7.1.113
0.006
-
UTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
0.0065
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP; pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
2.7.1.113
0.01
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
2.7.1.113
0.0104
-
2'-deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
2.7.1.113
0.017
-
2'-deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
2.7.1.113
0.035
-
CTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
0.036
-
ATP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
0.046
-
GTP
pH 8.5, 37°C; pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Mg2+
required for activity
Bacillus subtilis
2.7.1.113
Mg2+
MgNTP2- is most probably the true phosphate donor; required for activity; required for activity, no inhibition by excess Mg2+, at 5 mM
Bacillus subtilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.113
24147
-
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
2.7.1.113
49000
-
recombinant deoxyguanosine kinase, gel filtration; recombinant enzyme, gel filtration
Bacillus subtilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.113
recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose; recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
2.7.1.113
0.54
-
enzyme in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyguanosine
Bacillus subtilis
2.7.1.113
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
2.7.1.113
8.1
-
purified recombinant enzyme, substrate 2'-deoxyguanosine; recombinant deoxyguanosine kinase
Bacillus subtilis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.113
-20°C, more than 1 mg/ml protein, 50% glycerol, 1 year, no loss of activity
Bacillus subtilis
2.7.1.113
-20°C, purified recombinant enzyme at 1 mg/ml, Tris-HCl, pH 7.8, 50% v/v glycerol, at least 1 year without loss of activity
Bacillus subtilis
2.7.1.113
0°C, the diluted enzyme is highly unstable, but in presence of 1 mg/ml bovine serum albumin, the diluted enzyme is stable for months
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
GTP + 2'-deoxyadenosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.76
GTP + 2'-deoxycytidine
-
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
additional information
substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine
640561
Bacillus subtilis
?
-
-
-
-
2.7.1.113
ATP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + deoxyadenosine
3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dAMP
-
-
-
?
2.7.1.113
ATP + deoxycytidine
recombinant deoxyguanosine kinase, 3% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dCMP
-
-
-
?
2.7.1.113
ATP + deoxyinosine
63% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + dIMP
-
640561
Bacillus subtilis
?
2.7.1.113
ATP + guanosine
recombinant deoxyguanosine kinase, 7% of activity with deoxyguanosine
640561
Bacillus subtilis
ADP + GMP
-
640561
Bacillus subtilis
?
2.7.1.113
CTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
CDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxyadenosine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxycytidine
very low activity
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxyguanosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + 2'-deoxyinosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.113
GTP + guanosine
very low activity
640561
Bacillus subtilis
GDP + guanosine 5'-phosphate
-
-
-
?
2.7.1.113
additional information
substrate specificity, the enzyme utilizes all naturally occurring ribo-and deoxyribonucleosides triphosphates, with the exception of dGTP, as phosphate donors, and shows poor activity with thymidine, inosine, adenosine, 2,3-dideoxyguanosine, 2,3-dideoxyinosine, 2,3-dideoxyadenosine, and hypoxanthine arabinoside as acceptor substrates
640561
Bacillus subtilis
?
-
-
-
-
2.7.1.113
UTP + 2'-deoxyguanosine
preferred substrates
640561
Bacillus subtilis
UDP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.113
dimer
2 * 24147, electrospray mass spectrometry; 2 * 24147, recombinant enzyme, electrospray mass spectrometry
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Bacillus subtilis
2.7.1.113
37
-
assay at
Bacillus subtilis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.113
1.41
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with UTP as donor substrate
Bacillus subtilis
2.7.1.113
1.41
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate UTP
Bacillus subtilis
2.7.1.113
1.41
-
UTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
1.53
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with ATP as donor substrate
Bacillus subtilis
2.7.1.113
1.53
-
ATP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
1.53
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate ATP
Bacillus subtilis
2.7.1.113
1.69
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with GTP as donor substrate
Bacillus subtilis
2.7.1.113
1.69
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate GTP
Bacillus subtilis
2.7.1.113
1.69
-
GTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
2.25
-
2'deoxyguanosine
pH 8.5, 37°C, recombinant enzyme, with CTP as donor substrate
Bacillus subtilis
2.7.1.113
2.25
-
CTP
pH 8.5, 37°C, recombinant enzyme, with 2'-deoxyguanosine as acceptor substrate
Bacillus subtilis
2.7.1.113
2.25
-
deoxyguanosine
pH 8.5, 37°C, cosubstrate CTP
Bacillus subtilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.76
8.5
-
assay at
Bacillus subtilis
2.7.1.113
9
-
; broad optimum
Bacillus subtilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.113
5.6
9
more than 80% of maximal activity at pH 7.5 and pH 11.5, respectively, 60% of maximal activity at pH 6.0, complete loss of activity below pH 5.6
Bacillus subtilis
2.7.1.113
6
11.5
over 80% of maximal activity at pH 7.5 and pH 11.5, about 60% of maximal activity at pH 6.0, enzyme is completely inactive below pH 5.6
Bacillus subtilis