BRENDA - Enzyme Database show

Substrate specificity of human recombinant mitochondrial deoxyguanosine kinase with cytostatic and antiviral purine and pyrimidine analogs

Sjoberg, A.H.; Wang, L.; Eriksson, S.; Mol. Pharmacol. 53, 270-273 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.113
expression in Escherichia coli
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.004
-
deoxyguanosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.012
-
deoxyinosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.033
-
arabinosyl guanine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.056
-
2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.078
-
2-chloro-2'-deoxyadenosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.34
-
deoxycytidine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.46
-
2-fluoro-arabinosyl-adenine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.47
-
deoxyadenosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.113
ATP + deoxyinosine
Homo sapiens
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
ADP + dIMP
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.113
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.113
recombinant enzyme, metal affinity chromatography
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.113
0.04
0.05
recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.113
ATP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + 2-chloro-2'-deoxyadenosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + 2-chloro-2'-deoxyadenosine monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + 2-fluoro-arabinosyl-adenine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + 2-fluoro-arabinosyl-adenine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + arabinosyl adenine
recombinant mitochondrial deoxyguanosine kinase, very low activity
640555
Homo sapiens
ADP + arabinosyl adenine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + arabinosyl guanine
recombinant mitochondrial deoxyguanosine kinase, better substrate than deoxyguanosine kinase
640555
Homo sapiens
ADP + arabinosyl guanine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxyadenosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + dAMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxycytidine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + dCMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxyinosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + dIMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxyinosine
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
640555
Homo sapiens
ADP + dIMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + dideoxyinosine
recombinant mitochondrial deoxyguanosine kinase, very low activity
640555
Homo sapiens
ADP + dideoxyinosine 5'-monophosphate
-
640555
Homo sapiens
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.113
expression in Escherichia coli
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.004
-
deoxyguanosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.012
-
deoxyinosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.033
-
arabinosyl guanine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.056
-
2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.078
-
2-chloro-2'-deoxyadenosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.34
-
deoxycytidine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.46
-
2-fluoro-arabinosyl-adenine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
2.7.1.113
0.47
-
deoxyadenosine
pH 7.6, recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.113
ATP + deoxyinosine
Homo sapiens
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
ADP + dIMP
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.113
recombinant enzyme, metal affinity chromatography
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.113
0.04
0.05
recombinant mitochondrial deoxyguanosine kinase
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.113
ATP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + 2-chloro-2'-deoxyadenosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + 2-chloro-2'-deoxyadenosine monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + 2-fluoro-arabinosyl-adenine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + 2-fluoro-arabinosyl-adenine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + arabinosyl adenine
recombinant mitochondrial deoxyguanosine kinase, very low activity
640555
Homo sapiens
ADP + arabinosyl adenine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + arabinosyl guanine
recombinant mitochondrial deoxyguanosine kinase, better substrate than deoxyguanosine kinase
640555
Homo sapiens
ADP + arabinosyl guanine 5'-monophosphate
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxyadenosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + dAMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxycytidine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + dCMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxyinosine
recombinant mitochondrial deoxyguanosine kinase
640555
Homo sapiens
ADP + dIMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + deoxyinosine
recombinant mitochondrial deoxyguanosine kinase, may be the preferred substrate in vivo
640555
Homo sapiens
ADP + dIMP
-
640555
Homo sapiens
?
2.7.1.113
ATP + dideoxyinosine
recombinant mitochondrial deoxyguanosine kinase, very low activity
640555
Homo sapiens
ADP + dideoxyinosine 5'-monophosphate
-
640555
Homo sapiens
?