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Literature summary extracted from

  • Wang, X.; Kemp, R.G.
    Identification of residues of Escherichia coli phosphofructokinase that contribute to nucleotide binding and specificity (1999), Biochemistry, 38, 4313-4318.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.11 F76A 62fold increase in Km for ATP Escherichia coli
2.7.1.11 F76W apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 F76Y apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R111E apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R11A apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R77A apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R77D apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R77E apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R77L apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 R82A 20fold increase in Km for ATP Escherichia coli
2.7.1.11 R82E apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 Y41A 49fold increase in Km for ATP Escherichia coli
2.7.1.11 Y41F apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 Y41L apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli
2.7.1.11 Y41W apparant Kms for ATP, ITP, GTP, CTP and UTP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.21
-
 ATP pH 7.2, 30°C Escherichia coli
2.7.1.11 2.2
-
 ITP pH 7.2, 30°C Escherichia coli
2.7.1.11 4.3
-
 GTP pH 7.2, 30°C Escherichia coli
2.7.1.11 5.1
-
 UTP pH 7.2, 30°C Escherichia coli
2.7.1.11 11
-
 CTP pH 7.2, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Escherichia coli P0A796
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 CTP + D-fructose 6-phosphate
-
 Escherichia coli CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Escherichia coli GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate
-
 Escherichia coli IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate
-
 Escherichia coli UDP + D-fructose 1,6-bisphosphate
-
 ?