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Literature summary extracted from

  • Turner, W.L.; Plaxton, W.C.
    Purification and characterization of pyrophosphate- and ATP-dependent phosphofructokinases from banana fruit (2003), Planta, 217, 113-121.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 D-fructose 1,6-diphosphate up to 24fold enhancement of glycolytic enzyme activity Musa cavendishii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 1,3-diphosphoglycerate 0.385 mM and 0.005 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition Musa acuminata
2.7.1.11 2-phosphoglycerate 0.032 mM and 0.004 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition Musa acuminata
2.7.1.11 ADP 1.9 mM and 0.0006 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition Musa acuminata
2.7.1.11 phosphoenolpyruvate 0.0023 mM and 0.0009 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition Musa acuminata
2.7.1.11 Phosphoglycolate 0.006 mM and 0.003 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition Musa acuminata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.021
-
 ATP pH 7.3, 25°C, PFK II Musa acuminata
2.7.1.11 0.03
-
 CTP pH 7.3, 25°C, PFK II Musa acuminata
2.7.1.11 0.032
-
 UTP pH 7.3, 25°C, PFK II Musa acuminata
2.7.1.11 0.034
-
 ATP pH 8.0, 25°C, PFK I Musa acuminata
2.7.1.11 0.035
-
 ITP pH 7.3, 25°C, PFK II Musa acuminata
2.7.1.11 0.038
-
 GTP pH 7.3, 25°C, PFK II Musa acuminata
2.7.1.11 0.055
-
 ATP
-
 Musa acuminata
2.7.1.11 0.055
-
 UTP pH 8.0, 25°C, PFK I Musa acuminata
2.7.1.11 0.082
-
 ITP pH 8.0, 25°C, PFK I Musa acuminata
2.7.1.11 0.099
-
 CTP pH 8.0, 25°C, PFK I Musa acuminata
2.7.1.90 0.0097
-
 diphosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
2.7.1.90 0.025
-
 D-fructose 1,6-diphosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
2.7.1.90 0.032
-
 D-fructose 6-phosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
2.7.1.90 0.082
-
 D-fructose 1,6-diphosphate pH 7.0, 25°C Musa cavendishii
2.7.1.90 0.093
-
 diphosphate pH 7.0, 25°C Musa cavendishii
2.7.1.90 0.38
-
 phosphate pH 7.0, 25°C Musa cavendishii
2.7.1.90 0.41
-
 phosphate pH 7.0, 25°C, presence of 0.005 mM D-fructose 1,6-diphosphate Musa cavendishii
2.7.1.90 1.1
-
 D-fructose 6-phosphate pH 7.0, 25°C Musa cavendishii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 160000
-
 PFK II, gel filtration Musa acuminata
2.7.1.11 210000
-
 PFK I, gel filtration Musa acuminata
2.7.1.90 60000
-
 4 * 66000, alpha-subunit, + 4 * 60000, beta-subunit, SDS-PAGE Musa cavendishii
2.7.1.90 66000
-
 4 * 66000, alpha-subunit, + 4 * 60000, beta-subunit, SDS-PAGE Musa cavendishii
2.7.1.90 490000
-
 gel filtration Musa cavendishii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Musa acuminata
-
 L. cv. Cavendish, banana, isoenzymes PFK I and PFK II
-
2.7.1.90 Musa cavendishii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 PFK I and PFK II, butyl-Sepharose, DEAE-Fractogel, Superose 6 Musa acuminata
2.7.1.90
-
 Musa cavendishii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 fruit ripened fruit Musa acuminata
-
2.7.1.90 fruit
-
 Musa cavendishii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 5.5
-
 PFK II Musa acuminata
2.7.1.11 6
-
 PFK I Musa acuminata
2.7.1.90 13.9
-
 pH 7.0, 25°C Musa cavendishii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Musa acuminata ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate
-
 Musa acuminata CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Musa acuminata GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate
-
 Musa acuminata IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate
-
 Musa acuminata UDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Musa cavendishii phosphate + D-fructose 1,6-diphosphate
-
 r
2.7.1.90 phosphate + D-fructose 1,6-diphosphate
-
 Musa cavendishii diphosphate + D-fructose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 octamer 4 * 66000, alpha-subunit, + 4 * 60000, beta-subunit, SDS-PAGE Musa cavendishii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7.3
-
 PFK II Musa acuminata
2.7.1.11 8
-
 PFK I Musa acuminata
2.7.1.90 7.1
-
 braod Musa cavendishii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 6.3 8.7 PFK II, approx. 30% of maximal activity at pH 6.5, approx. 10% of maximal activity at pH 8.6 Musa acuminata
2.7.1.11 6.5 9 PFK I, approx. 10% of maximal activity at pH 6.5, approx. 60% of maximal activity at pH 9.0 Musa acuminata