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Literature summary extracted from

  • Ding, Y.H.R.; Ronimus, R.S.; Morgan, H.W.
    Thermotoga maritima phosphofructokinases: expression and characterization of two unique enzymes (2001), J. Bacteriol., 183, 791-794.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 Cu2+ 0.001 mM, 28% inhibition Thermotoga maritima
2.7.1.11 diphosphate 0.1 mM, 80% inhibition, nucleotide diphosphates, i.e. ADP, GDP or TDP, partially alleviate inhibition Thermotoga maritima
2.7.1.11 Zn2+ 0.001 mM, 28% inhibition Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.009
-
 ATP pH 7.8, 50°C Thermotoga maritima
2.7.1.11 0.44
-
 D-fructose 6-phosphate pH 7.8, 50°C Thermotoga maritima
2.7.1.11 1.36
-
 GTP pH 7.8, 50°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Fe2+ 34% of activity with Mg2+ Thermotoga maritima
2.7.1.11 K+ required for activity, maximal activity at 175 mM, 50% lower activity in the absence of K+ Thermotoga maritima
2.7.1.11 Mg2+ required for activity Thermotoga maritima
2.7.1.11 Mn2+ 90% of activity with Mg2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 34447
-
 alpha4, 4 * 34447, deduced from nucleotide sequence Thermotoga maritima
2.7.1.11 200000
-
 recombinant PFK, gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 recombinant PFK Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 CTP + D-fructose 6-phosphate 13% of activity with ATP Thermotoga maritima CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate 42% of activity with ATP Thermotoga maritima GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 TTP + D-fructose 6-phosphate 10% of activity with ATP Thermotoga maritima TDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate 14% of activity with ATP Thermotoga maritima UDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 tetramer alpha4, 4 * 34447, deduced from nucleotide sequence Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.11 90
-
 half-life grater than 5 h in phosphate buffer Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7.2 8 fructose 6-phosphate phosphorylation Thermotoga maritima