BRENDA - Enzyme Database

Kinetic properties of ATP-dependent phosphofructokinase from grapefruit juice sacs: effect of TCA cycle intermediates

Van Praag, E.; Zehavi, U.; Goren, R.; Biochem. Mol. Biol. Int. 47, 749-756 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.11
2-oxoglutarate
competitive inhibition
Citrus x paradisi
2.7.1.11
ATP
inhibition above 0.08 mM
Citrus x paradisi
2.7.1.11
citrate
presence of 12 mM Mg2+ relieves inhibition completely
Citrus x paradisi
2.7.1.11
isocitrate
competitive inhibition
Citrus x paradisi
2.7.1.11
malate
competitive inhibition
Citrus x paradisi
2.7.1.11
succinate
competitive inhibition
Citrus x paradisi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.11
0.04
-
ATP
pH 7.5
Citrus x paradisi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.11
ATP + D-fructose 6-phosphate
Citrus x paradisi
-
ADP + D-fructose 1,6-bisphosphate
-
Citrus x paradisi
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.11
Citrus x paradisi
-
Macf, cv. Marsh, grapefruit
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.11
pulp
-
Citrus x paradisi
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.11
ATP + D-fructose 6-phosphate
-
640507
Citrus x paradisi
ADP + D-fructose 1,6-bisphosphate
-
640507
Citrus x paradisi
?
2.7.1.11
ATP + D-fructose 6-phosphate
fructose 6-phosphate shows negative cooperativity at low concentrations and positive cooperativity at high concentrations
640507
Citrus x paradisi
ADP + D-fructose 1,6-bisphosphate
-
640507
Citrus x paradisi
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.11
1.3
-
citrate
pH 7.5, theoretical value
Citrus x paradisi
2.7.1.11
1.43
-
malate
pH 7.5
Citrus x paradisi
2.7.1.11
3.21
-
succinate
pH 7.5
Citrus x paradisi
2.7.1.11
3.57
-
isocitrate
pH 7.5
Citrus x paradisi
2.7.1.11
3.9
-
2-oxoglutarate
pH 7.5
Citrus x paradisi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.11
2-oxoglutarate
competitive inhibition
Citrus x paradisi
2.7.1.11
ATP
inhibition above 0.08 mM
Citrus x paradisi
2.7.1.11
citrate
presence of 12 mM Mg2+ relieves inhibition completely
Citrus x paradisi
2.7.1.11
isocitrate
competitive inhibition
Citrus x paradisi
2.7.1.11
malate
competitive inhibition
Citrus x paradisi
2.7.1.11
succinate
competitive inhibition
Citrus x paradisi
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.11
1.3
-
citrate
pH 7.5, theoretical value
Citrus x paradisi
2.7.1.11
1.43
-
malate
pH 7.5
Citrus x paradisi
2.7.1.11
3.21
-
succinate
pH 7.5
Citrus x paradisi
2.7.1.11
3.57
-
isocitrate
pH 7.5
Citrus x paradisi
2.7.1.11
3.9
-
2-oxoglutarate
pH 7.5
Citrus x paradisi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.11
0.04
-
ATP
pH 7.5
Citrus x paradisi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.11
ATP + D-fructose 6-phosphate
Citrus x paradisi
-
ADP + D-fructose 1,6-bisphosphate
-
Citrus x paradisi
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.11
pulp
-
Citrus x paradisi
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.11
ATP + D-fructose 6-phosphate
-
640507
Citrus x paradisi
ADP + D-fructose 1,6-bisphosphate
-
640507
Citrus x paradisi
?
2.7.1.11
ATP + D-fructose 6-phosphate
fructose 6-phosphate shows negative cooperativity at low concentrations and positive cooperativity at high concentrations
640507
Citrus x paradisi
ADP + D-fructose 1,6-bisphosphate
-
640507
Citrus x paradisi
?