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Literature summary extracted from

  • Massey, T.; Deal, W.C.
    Phosphofructokinase from porcine liver and kidney and from other mammalian tissues (1975), Methods Enzymol., 42C, 99-110.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.11
-
 Gallus gallus
2.7.1.11
-
 Sus scrofa
2.7.1.11
-
 Oryctolagus cuniculus
2.7.1.11
-
 Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP
-
 Bos taurus
2.7.1.11 ATP
-
 Homo sapiens
2.7.1.11 ATP
-
 Oryctolagus cuniculus
2.7.1.11 ATP
-
 Ovis aries
2.7.1.11 ATP 1 mM, 90% inhibition of the activity observed with 0.1 mM ATP Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.01
-
 ATP pH 7.0 Sus scrofa
2.7.1.11 0.023
-
 D-fructose 6-phosphate pH 7.0 and pH 8.0 Sus scrofa
2.7.1.11 0.025
-
 ATP pH 8.0 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 3500000
-
 above, sedimentation velocity studies, concentration dependent molecular weight Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Bos taurus
-
-
-
2.7.1.11 Capra hircus
-
-
-
2.7.1.11 Gallus gallus
-
-
-
2.7.1.11 Homo sapiens
-
-
-
2.7.1.11 Oryctolagus cuniculus
-
-
-
2.7.1.11 Ovis aries
-
-
-
2.7.1.11 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 heart
-
 Bos taurus
-
2.7.1.11 heart
-
 Oryctolagus cuniculus
-
2.7.1.11 heart
-
 Ovis aries
-
2.7.1.11 kidney
-
 Oryctolagus cuniculus
-
2.7.1.11 liver
-
 Gallus gallus
-
2.7.1.11 liver
-
 Homo sapiens
-
2.7.1.11 liver
-
 Sus scrofa
-
2.7.1.11 liver
-
 Oryctolagus cuniculus
-
2.7.1.11 liver
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 100
-
 liver PFK Sus scrofa
2.7.1.11 128
-
 kidney PFK Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 more stable if kept under nitrogen or with dithiothreitol Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Gallus gallus ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Homo sapiens ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Sus scrofa ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Bos taurus ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Oryctolagus cuniculus ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Ovis aries ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Capra hircus ADP + D-fructose 1,6-bisphosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.11 0.25
-
 ATP pH 7.0 Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.11 Sus scrofa isoelectric focusing
-
 5