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Literature summary extracted from

  • Byrnes, M.; Zhu, X.; Younathan, E.S.; Chang, S.H.
    Kinetic characteristics of phosphofructokinase from Bacillus stearothermophilus: MgATP nonallosterically inhibits the enzyme (1994), Biochemistry, 33, 3424-3431.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expression in Escherichia coli Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.11 G212V inhibition by phosphoenolpyruvate can not be reversed by GDP or ADP, neither ADP nor GDP bind well to the effector site Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 MgATP2-
-
 Geobacillus stearothermophilus
2.7.1.11 additional information product inhibition of reverse reaction Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information kinetic study Geobacillus stearothermophilus
2.7.1.11 0.028
-
 D-fructose 6-phosphate pH 8.2, 30°C, cosubstrate GTP Geobacillus stearothermophilus
2.7.1.11 0.03
-
 D-fructose 6-phosphate pH 8.2, 30°C, at fructose 6-phosphate concentrations that are less than approx. one-third the fixed MgATP concentration Geobacillus stearothermophilus
2.7.1.11 0.068
-
 D-fructose 6-phosphate pH 8.2, 30°C, cosubstrate UTP Geobacillus stearothermophilus
2.7.1.11 0.07
-
 ATP pH 8.2, 30°C Geobacillus stearothermophilus
2.7.1.11 0.15
-
 D-fructose 6-phosphate pH 8.2, 30°C, cosubstrate CTP Geobacillus stearothermophilus
2.7.1.11 0.18
-
 GTP pH 8.2, 30°C Geobacillus stearothermophilus
2.7.1.11 2.6
-
 CTP pH 8.2, 30°C Geobacillus stearothermophilus
2.7.1.11 2.8
-
 UTP pH 8.2, 30°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Geobacillus stearothermophilus
-
 expressed in PFK-deficient Escherichia coli DF1020 cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.11 ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate mechanism Geobacillus stearothermophilus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 160
-
-
 Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 -20°C, in 100 mM Tris-HCl buffer, pH 7.4, 1 mM dithiothreitol, 50% glycerol, stable Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate 35fold lower reverse reaction velocity Geobacillus stearothermophilus ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 CTP + D-fructose 6-phosphate
-
 Geobacillus stearothermophilus CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Geobacillus stearothermophilus GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate
-
 Geobacillus stearothermophilus UDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 30
-
 assay at Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.11 3.2
-
 fructose 1,6-bisphosphate reverse raction, recombinant enzyme Geobacillus stearothermophilus
2.7.1.11 112
-
 fructose 6-phosphate forward reaction, recombinant enzyme Geobacillus stearothermophilus