EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.11 | ATP | above 1 mM at 0.33 mM fructose 6-phosphate and 2 mM Mg2+, inhibition at a Mg/ATP ratio below 2; ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; Mg2+ partially reverses inhibition | Klebsiella aerogenes | |
2.7.1.11 | citrate | cAMP, ADP or fructose 1,6-bisphosphate restore activity | Klebsiella aerogenes | |
2.7.1.11 | CTP | - |
Klebsiella aerogenes | |
2.7.1.11 | GTP | - |
Klebsiella aerogenes | |
2.7.1.11 | additional information | not inhibited by ADP | Klebsiella aerogenes | |
2.7.1.11 | nucleoside triphosphate | at a Mg/NTP ratio below 2 | Klebsiella aerogenes | |
2.7.1.11 | UTP | - |
Klebsiella aerogenes | |
2.7.1.56 | ATP | additional Mg2+ reverses; at a Mg2+/ATP ratio below 2 | Klebsiella aerogenes | |
2.7.1.56 | citrate | - |
Klebsiella aerogenes | |
2.7.1.56 | D-fructose 1,6-bisphosphate | - |
Klebsiella aerogenes | |
2.7.1.56 | D-fructose 6-phosphate | - |
Klebsiella aerogenes | |
2.7.1.56 | GTP | at a Mg2+/GTP ratio below 2 | Klebsiella aerogenes | |
2.7.1.56 | ITP | at a Mg2+/ITP ratio below 2 | Klebsiella aerogenes | |
2.7.1.56 | additional information | not inhibited by ATP; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by nucleoside monophosphates or diphosphates, D-mannose 6-phosphate, D-fructose, L-fructose 1-phosphate; not inhibited by phosphate, AMP | Klebsiella aerogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.11 | 0.3 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 2/1 | Klebsiella aerogenes | |
2.7.1.11 | 0.6 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 1.4/1 | Klebsiella aerogenes | |
2.7.1.11 | 1.4 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 0.7/1 | Klebsiella aerogenes | |
2.7.1.56 | 0.7 | - |
ATP | - |
Klebsiella aerogenes | |
2.7.1.56 | 0.75 | - |
D-Fructose 1-phosphate | - |
Klebsiella aerogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.56 | Mg2+ | required for activity | Klebsiella aerogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.11 | 90000 | - |
gel filtration | Klebsiella aerogenes |
2.7.1.56 | 75000 | - |
gel filtration | Klebsiella aerogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.11 | Klebsiella aerogenes | - |
strains PRL-R3, wild-type, and its mutant DD31 | - |
2.7.1.56 | Klebsiella aerogenes | - |
wild-type strain PRL-R3 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.11 | protamine sulfate, ammonium sulfate, Sephadex G-200, partial purification | Klebsiella aerogenes |
2.7.1.56 | - |
Klebsiella aerogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.11 | 0.75 | - |
- |
Klebsiella aerogenes |
2.7.1.56 | 47 | - |
- |
Klebsiella aerogenes |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.56 | -20°C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months | Klebsiella aerogenes |
2.7.1.56 | -20°C, crude enzyme extract prepared in water, at least 3 weeks | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.11 | ATP + D-fructose 6-phosphate | - |
Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | ATP + D-fructose 1-phosphate | no activity with D-fructose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | ATP + D-fructose 1-phosphate | no activity with D-glucose 6-phosphate, D-glucose 1-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | ATP + D-fructose 1-phosphate | specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | CTP + D-fructose 1-phosphate | poor substrate | Klebsiella aerogenes | CDP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | GTP + D-fructose 1-phosphate | 35% of activity with ATP | Klebsiella aerogenes | GDP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | ITP + D-fructose 1-phosphate | 43% of activity with ATP | Klebsiella aerogenes | IDP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | TTP + D-fructose 1-phosphate | poor substrate | Klebsiella aerogenes | TDP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.56 | UTP + D-fructose 1-phosphate | poor substrate | Klebsiella aerogenes | UDP + D-fructose 1,6-bisphosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.11 | 25 | - |
assay at | Klebsiella aerogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.11 | 8 | - |
- |
Klebsiella aerogenes |
2.7.1.56 | 7.5 | - |
- |
Klebsiella aerogenes |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.11 | 7 | 9 | approx. 80% of maximal activity at pH 7 and pH 9, approx. half-maximal activity at pH 6.7 | Klebsiella aerogenes |
2.7.1.56 | 7 | 8.7 | approx. 80% of maximal activity at pH 7. approx. half-maximal activity at pH 8.7 | Klebsiella aerogenes |