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Literature summary extracted from

  • Sapico, V.; Anderson, R.L.
    D-fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties (1969), J. Biol. Chem., 244, 6280-6288.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP above 1 mM at 0.33 mM fructose 6-phosphate and 2 mM Mg2+, inhibition at a Mg/ATP ratio below 2; ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; Mg2+ partially reverses inhibition Klebsiella aerogenes
2.7.1.11 citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Klebsiella aerogenes
2.7.1.11 CTP
-
Klebsiella aerogenes
2.7.1.11 GTP
-
Klebsiella aerogenes
2.7.1.11 additional information not inhibited by ADP Klebsiella aerogenes
2.7.1.11 nucleoside triphosphate at a Mg/NTP ratio below 2 Klebsiella aerogenes
2.7.1.11 UTP
-
Klebsiella aerogenes
2.7.1.56 ATP additional Mg2+ reverses; at a Mg2+/ATP ratio below 2 Klebsiella aerogenes
2.7.1.56 citrate
-
Klebsiella aerogenes
2.7.1.56 D-fructose 1,6-bisphosphate
-
Klebsiella aerogenes
2.7.1.56 D-fructose 6-phosphate
-
Klebsiella aerogenes
2.7.1.56 GTP at a Mg2+/GTP ratio below 2 Klebsiella aerogenes
2.7.1.56 ITP at a Mg2+/ITP ratio below 2 Klebsiella aerogenes
2.7.1.56 additional information not inhibited by ATP; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by nucleoside monophosphates or diphosphates, D-mannose 6-phosphate, D-fructose, L-fructose 1-phosphate; not inhibited by phosphate, AMP Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.3
-
D-fructose 6-phosphate pH 8.0, 25, at a Mg2+/ATP ratio of 2/1 Klebsiella aerogenes
2.7.1.11 0.6
-
D-fructose 6-phosphate pH 8.0, 25, at a Mg2+/ATP ratio of 1.4/1 Klebsiella aerogenes
2.7.1.11 1.4
-
D-fructose 6-phosphate pH 8.0, 25, at a Mg2+/ATP ratio of 0.7/1 Klebsiella aerogenes
2.7.1.56 0.7
-
ATP
-
Klebsiella aerogenes
2.7.1.56 0.75
-
D-Fructose 1-phosphate
-
Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 Mg2+ required for activity Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 90000
-
gel filtration Klebsiella aerogenes
2.7.1.56 75000
-
gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Klebsiella aerogenes
-
strains PRL-R3, wild-type, and its mutant DD31
-
2.7.1.56 Klebsiella aerogenes
-
wild-type strain PRL-R3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 protamine sulfate, ammonium sulfate, Sephadex G-200, partial purification Klebsiella aerogenes
2.7.1.56
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 0.75
-
-
Klebsiella aerogenes
2.7.1.56 47
-
-
Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.56 -20°C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months Klebsiella aerogenes
2.7.1.56 -20°C, crude enzyme extract prepared in water, at least 3 weeks Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 ATP + D-fructose 1-phosphate no activity with D-glucose 6-phosphate, D-glucose 1-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 ATP + D-fructose 1-phosphate specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 CTP + D-fructose 1-phosphate poor substrate Klebsiella aerogenes CDP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 GTP + D-fructose 1-phosphate 35% of activity with ATP Klebsiella aerogenes GDP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 ITP + D-fructose 1-phosphate 43% of activity with ATP Klebsiella aerogenes IDP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 TTP + D-fructose 1-phosphate poor substrate Klebsiella aerogenes TDP + D-fructose 1,6-bisphosphate
-
?
2.7.1.56 UTP + D-fructose 1-phosphate poor substrate Klebsiella aerogenes UDP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 25
-
assay at Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 8
-
-
Klebsiella aerogenes
2.7.1.56 7.5
-
-
Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 7 9 approx. 80% of maximal activity at pH 7 and pH 9, approx. half-maximal activity at pH 6.7 Klebsiella aerogenes
2.7.1.56 7 8.7 approx. 80% of maximal activity at pH 7. approx. half-maximal activity at pH 8.7 Klebsiella aerogenes