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Literature summary extracted from

  • Kemp, R.G.
    Phosphofructokinase from rabbit liver (1975), Methods Enzymol., 42C, 67-71.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 NH4+
-
 Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.1.11 fructose 6-phosphate, fructose 1,6-bisphosphate and ammonium sulfate stabilize dilute enzyme solutions Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 2,3-diphosphoglycerate
-
 Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 liver PFK, ammonium sulfate, DEAE-Sephadex, agarose chromatography Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 85 95
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 -20°C, more than 2 mg protein per ml, in 50 mM Tris-phosphate buffer, pH 8, 2 mM EDTA, 100 mM ammonium sulfate, 0.1 mM ATP, 0.5 mM dithiothreitol, several months Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Oryctolagus cuniculus ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 26
-
 assay at Oryctolagus cuniculus