BRENDA - Enzyme Database

Phosphofructokinase from oyster adductor muscle

Storey, K.B.; Methods Enzymol. 90, 39-44 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.11
AMP
activation
Crassostrea virginica
General Stability
EC Number
General Stability
Organism
2.7.1.11
polyethylene glycol stabilizes
Crassostrea virginica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.11
arginine phosphate
-
Crassostrea virginica
2.7.1.11
citrate
cAMP, ADP or fructose 1,6-bisphosphate restore activity
Crassostrea virginica
2.7.1.11
additional information
not inhibited by ADP; not inhibited by Ca2+; not inhibited by fructose 1,6-bisphosphate
Crassostrea virginica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.11
0.01
-
Mg2+
pH 7.9, 24°C
Crassostrea virginica
2.7.1.11
0.05
-
ATP
pH 7.9, 24°C
Crassostrea virginica
2.7.1.11
0.5
-
D-fructose 6-phosphate
pH 7.9, 24°C
Crassostrea virginica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.11
Mg2+
Km: 0.01 mM; required for activity
Crassostrea virginica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.11
340000
-
-
Crassostrea virginica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.11
Crassostrea virginica
-
oyster
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.11
polyethylene glycol, ATP-agarose, Sephacryl S-300
Crassostrea virginica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.11
146.7
-
-
Crassostrea virginica
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.11
4°C, t1/2: 1.5-2 days, ATP and/or fructose 1,6-bisphosphate increase stability
Crassostrea virginica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.11
ATP + D-fructose 6-phosphate
-
640460
Crassostrea virginica
ADP + D-fructose 1,6-bisphosphate
-
640460
Crassostrea virginica
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.11
24
-
assay at
Crassostrea virginica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.11
7.9
-
-
Crassostrea virginica
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.11
AMP
activation
Crassostrea virginica
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.11
polyethylene glycol stabilizes
Crassostrea virginica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.11
arginine phosphate
-
Crassostrea virginica
2.7.1.11
citrate
cAMP, ADP or fructose 1,6-bisphosphate restore activity
Crassostrea virginica
2.7.1.11
additional information
not inhibited by ADP; not inhibited by Ca2+; not inhibited by fructose 1,6-bisphosphate
Crassostrea virginica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.11
0.01
-
Mg2+
pH 7.9, 24°C
Crassostrea virginica
2.7.1.11
0.05
-
ATP
pH 7.9, 24°C
Crassostrea virginica
2.7.1.11
0.5
-
D-fructose 6-phosphate
pH 7.9, 24°C
Crassostrea virginica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.11
Mg2+
Km: 0.01 mM; required for activity
Crassostrea virginica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.11
340000
-
-
Crassostrea virginica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.11
polyethylene glycol, ATP-agarose, Sephacryl S-300
Crassostrea virginica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.11
146.7
-
-
Crassostrea virginica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.11
4°C, t1/2: 1.5-2 days, ATP and/or fructose 1,6-bisphosphate increase stability
Crassostrea virginica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.11
ATP + D-fructose 6-phosphate
-
640460
Crassostrea virginica
ADP + D-fructose 1,6-bisphosphate
-
640460
Crassostrea virginica
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.11
24
-
assay at
Crassostrea virginica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.11
7.9
-
-
Crassostrea virginica