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Literature summary extracted from

  • Hofer, H.W.; Allen, B.L.; Kaeini, M.R.; Pette, D.; Harris, B.G.
    Phosphofructokinase from Ascaris suum. Regulatory kinetic studies and activity near physiological conditions (1982), J. Biol. Chem., 257, 3801-3806.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 D-fructose 1,6-bisphosphate activation Ascaris suum
2.7.1.11 D-Glucose 1,6-bisphosphate
-
 Ascaris suum
2.7.1.11 additional information effector studies at near-physiological conditions Ascaris suum
2.7.1.11 NH4+ at very low concentration, kinetics, Km-value: 0.56 mM Ascaris suum
2.7.1.11 phosphate kinetics, Km-value: 1.64 mM Ascaris suum
2.7.1.11 SO42- stimulation Ascaris suum
2.7.1.11 SO42- kinetics, Km-value: 1.73 mM Ascaris suum

General Stability

EC Number General Stability Organism
2.7.1.11 unstable in buffers like imidazole-HCl, pH 6.6 or 6.8, or Tris-HCl, pH 8, 50 mM phosphate and 0.3 M KCl stabilize Ascaris suum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP fructose 6-phosphate partially reverses inhibition Ascaris suum
2.7.1.11 citrate weak inhibition Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information kinetic study Ascaris suum
2.7.1.11 additional information
-
 additional information kinetic properties of phosphorylated enzyme Ascaris suum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 K+ kinetics, Km-value: 11.1 mM Ascaris suum
2.7.1.11 Mg2+ required for activity Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Ascaris suum
-
 female parasitic nematode, from small intestine of pig
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 muscle
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 49.6
-
-
 Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Ascaris suum ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 30
-
 assay at Ascaris suum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.11 Ascaris suum isoelectric focusing
-
 7.4