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Literature summary extracted from

  • Botha, F.C.; Turpin, D.H.
    Molecular, kinetic, and immunological properties of the 6-phosphofructokinase from the green alga Selenastrum minutum (1990), Plant Physiol., 93, 871-879.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 phosphate 25% activation at 5 mM, inhibition above Monoraphidium minutum

General Stability

EC Number General Stability Organism
2.7.1.11 at least 15 mM phosphate required to stabilize PFK during purification, presence of 10% glycerol helps to stabilize Monoraphidium minutum
2.7.1.11 largest molecular weight-form of purified enzyme is the most stable form Monoraphidium minutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 2-oxoglutarate 2 mM, 92% inhibition Monoraphidium minutum
2.7.1.11 3-phosphoglycerate 2 mM, 86% inhibition; inhibition kinetics Monoraphidium minutum
2.7.1.11 ATP above 0.2 mM, inhibition is alleviated by Mg2+; free form Monoraphidium minutum
2.7.1.11 citrate 2 mM, complete inhibition Monoraphidium minutum
2.7.1.11 malate 2 mM, 92% inhibition Monoraphidium minutum
2.7.1.11 additional information not inhibited by 2-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by glutamine, glutamate Monoraphidium minutum
2.7.1.11 phosphoenolpyruvate 2 mM, complete inhibition; inhibition kinetic Monoraphidium minutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
additional information kinetic study Monoraphidium minutum
2.7.1.11 0.009
-
ATP pH 7.25, 25°C Monoraphidium minutum
2.7.1.11 0.01
-
ATP
-
Monoraphidium minutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.11 plastid
-
Monoraphidium minutum 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ Km: 0.5 mM Monoraphidium minutum
2.7.1.11 Mg2+ required for activity Monoraphidium minutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 70000
-
4 * 70000, SDS-PAGE, smallest catalytically active form Monoraphidium minutum
2.7.1.11 280000
-
gel filtration, smallest catalytically active form, tends to aggregate to different active forms Monoraphidium minutum
2.7.1.11 6000000
-
gel filtration, largest catalytically active form, tends to aggregate to different active forms Monoraphidium minutum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Monoraphidium minutum
-
green alga
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 no isozymes Monoraphidium minutum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 122 180
-
Monoraphidium minutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
Monoraphidium minutum ADP + D-fructose 1,6-bisphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 tetramer 4 * 70000, SDS-PAGE, smallest catalytically active form Monoraphidium minutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7.3
-
-
Monoraphidium minutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 6.5 8.5 approx. half-maximal activity at pH 6.5 and pH 8.2 Monoraphidium minutum