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Literature summary extracted from

  • Kombrink, E.; Wober, G.
    Chloroplast phosphofructokinase in the green alga, Dunaliella marina: partial purification and kinetic and regulatory properties (1982), Arch. Biochem. Biophys., 213, 602-619.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 phosphate kinetics Dunaliella marina
2.7.1.11 phosphate the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio Dunaliella marina

General Stability

EC Number General Stability Organism
2.7.1.11 very unstable upon purification, dithioerythritol, K+ or Mg2+ and phosphate partially stabilize Dunaliella marina

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 2-phosphoglycerate 1 mM, complete inhibition, phosphate relieves, kinetics Dunaliella marina
2.7.1.11 3-phosphoglycerate 1 mM, complete inhibition, phosphate relieves, 0.5 mM, 50% inhibition, sigmoidal inhibition curve; inhibition kinetics Dunaliella marina
2.7.1.11 ADP
-
 Dunaliella marina
2.7.1.11 ATP strong inhibition above 1 mM, at 2.5 mM Mg2+ Dunaliella marina
2.7.1.11 citrate
-
 Dunaliella marina
2.7.1.11 D-glucose 6-phosphate weak inhibition Dunaliella marina
2.7.1.11 additional information not inhibited by ADPglucose, dithiothreitol, gluconate 6-phosphate, glucose 1-phosphate; not inhibited by NH4+ and K+ Dunaliella marina
2.7.1.11 phosphate above 5 mM; kinetics; the enzyme is both activated and inhibited by phosphate, depending on fructose 6-phosphate/phosphate-ratio Dunaliella marina
2.7.1.11 phosphoenolpyruvate 1 mM, complete inhibition, 0.08 mM, 50% inhibition; inhibition kinetic; phosphate relieves Dunaliella marina
2.7.1.11 Phosphoglycolate 1 mM, complete inhibition, phosphate relieves, kinetics Dunaliella marina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information kinetic study Dunaliella marina
2.7.1.11 0.06
-
 ATP pH 7.0 Dunaliella marina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.11 chloroplast exclusively Dunaliella marina 9507
-
2.7.1.11 plastid chloroplasts Dunaliella marina 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Dunaliella marina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 282000
-
 30 h sucrose density gradient centrifugation Dunaliella marina
2.7.1.11 434000
-
 15 h sucrose density gradient centrifugation Dunaliella marina
2.7.1.11 644000
-
 5 h sucrose density gradient centrifugation Dunaliella marina

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Dunaliella marina
-
 green alga
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 ammonium sulfate, DEAE-cellulose, partial purification Dunaliella marina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 0.38
-
-
 Dunaliella marina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Dunaliella marina ADP + D-fructose 1,6-bisphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7
-
-
 Dunaliella marina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 6 8.5 approx. half-maximal activity at pH 6 and pH 8.5 Dunaliella marina