Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hausler, R.E.; Holtum, J.A.M.; Latzko, E.
    Cytosolic phosphofructokinase from spinach leaves: 1. Purification and regulation by magnesium and phosphate (1989), Plant Physiol., 90, 1498-1505.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 citrate stimulation, by chelating of free Mg2+ Spinacia oleracea
2.7.1.11 Cl- 200 mM, 3.1fold activation of cytosolic PFK Spinacia oleracea
2.7.1.11 HAsO42- 50 mM, 2.9fold activation of cytosolic PFK Spinacia oleracea
2.7.1.11 HCO3- 200 mM, 2.1fold activation of cytosolic PFK Spinacia oleracea
2.7.1.11 NH4+ cytosolic isozyme is not activated Spinacia oleracea
2.7.1.11 NO3- 50 mM 1.6fold activation of cytosolic PFK Spinacia oleracea
2.7.1.11 phosphate 25 mM, 3.2fold activation of cytosolic PFK Spinacia oleracea
2.7.1.11 SO42- 50 mM, 2.6fold activation of cytosolic PFK Spinacia oleracea

General Stability

EC Number General Stability Organism
2.7.1.11 ATP stabilizes Spinacia oleracea
2.7.1.11 glycerol prevents inactivation of dilute cytosolic enzyme solution Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 2-phosphoglycerate 1.3 mM, 50% inhibition of cytosolic PFK, no inhibition in the presence of 5 or 25 mM phosphate Spinacia oleracea
2.7.1.11 diphosphate
-
 Spinacia oleracea
2.7.1.11 K+
-
 Spinacia oleracea
2.7.1.11 additional information not inhibited by 3-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by Na+ Spinacia oleracea
2.7.1.11 phosphoenolpyruvate 0.22 mM, 50% inhibition of cytosolic PFK in the presence of 1 mM fructose 6-phosphate, phosphate relives inhibition Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.11 chloroplast
-
 Spinacia oleracea 9507
-
2.7.1.11 cytosol cytosolic and plastidic isoenzyme Spinacia oleracea 5829
-
2.7.1.11 plastid chloroplasts Spinacia oleracea 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Spinacia oleracea
2.7.1.11 Mg2+ MgATP is the active substrate Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 cytosolic PFK, ammonium sulfate, DEAE-Sephacel, Blue Sepharose, ATP-agarose Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 20.5
-
 cytosolic PFK Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 -20°C, 50 mM Tricine-NaOH buffer, pH 7.9, 50% v/v glycerol, at least 2 months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Spinacia oleracea ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 25
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 additional information
-
 pH-dependence is influenced by Mg2+ and/or phosphate Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.11 0.41
-
 diphosphate pH 7.7, 25°C Spinacia oleracea