Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cawood, M.E.; Botha, F.C.; Small, J.G.C.
    Properties of the phosphofructokinase isoenzymes from germinating cucumber seeds (1988), J. Plant Physiol., 132, 204-209.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 NH4+ 40 mM, 50% activation at pH 8.0 and pH 7.2 Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 2-phosphoglycerate 2 mM and 3.2 mM, 50% inhibition of cytosolic PFK at pH 8.0 and PH 7.2, respectively, 0.5 mM and 0.4 mM, 50% inhibition of plastid PFK at pH 8.0 and pH 7.2, respectively Cucumis sativus
2.7.1.11 3-phosphoglycerate
-
 Cucumis sativus
2.7.1.11 ADP most potent inhibitor of cytosolic isozyme, 0.4 mM and 4 mM, 50% inhibition of cytosolic and plastid PFK at pH 8.0 Cucumis sativus
2.7.1.11 ATP 2 mM, 50% inhibition of cytosolic PFK at pH 8.0, 5 mM, 50% inhibition of plastid PFK at pH 8.0 Cucumis sativus
2.7.1.11 GTP plastid, not cytosolic, isozyme Cucumis sativus
2.7.1.11 additional information cytosolic PFK is not inhibited by GTP; not inhibited by fructose 2,6-bisphosphate Cucumis sativus
2.7.1.11 nucleoside triphosphate
-
 Cucumis sativus
2.7.1.11 phosphoenolpyruvate 0.04 mM and 0.05 mM, 50% inhibition of plastid PFK at pH 8.0 and pH 7.2, respectively; most potent inhibitor Cucumis sativus
2.7.1.11 SO42- 20 mM, 50% inhibition of cytosolic and 77% inhibition of plastid PFK at pH 7.2, not at pH 8.0 Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information pH-dependence of kinetic properties of cytosolic and plastid isozymes Cucumis sativus
2.7.1.11 0.0006
-
 GTP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.0009
-
 GTP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.003
-
 ITP pH 8.0, 25°C, pH 7.2, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.004
-
 ITP pH 7.2, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.005
-
 ATP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.006
-
 ATP pH 7.2, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.007
-
 ATP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.008
-
 GTP pH 7.2, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.009
-
 UTP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.01
-
 UTP pH 8.0, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.011
-
 ITP pH 8.0, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.013
-
 GTP pH 8.0, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.014
-
 ATP pH 8.0, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.018
-
 CTP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.018
-
 UTP pH 8.0, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.019
-
 CTP pH 7.2, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.019
-
 UTP pH 7.2, 25°C, plastid PFK Cucumis sativus
2.7.1.11 0.021
-
 CTP pH 7.2, 25°C, cytosolic PFK Cucumis sativus
2.7.1.11 0.033
-
 CTP pH 8.0, 25°C, plastid PFK Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.11 cytosol cytosolic and plastidic isoenzyme Cucumis sativus 5829
-
2.7.1.11 plastid
-
 Cucumis sativus 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Cucumis sativus
-
 cucumber, cv. Fletcher
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 seed germinating Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 15
-
 cytosolic PFK Cucumis sativus
2.7.1.11 27
-
 plastid PFK Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate no activity with diphosphate as phosphate donor Cucumis sativus ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate
-
 Cucumis sativus CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Cucumis sativus GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate
-
 Cucumis sativus IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate
-
 Cucumis sativus UDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 25
-
 assay at Cucumis sativus