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Literature summary extracted from

  • Fukushima, E.; Sugiya, H.
    Purification and characterization of phosphofructokinase in bovine parotid gland (1992), Int. J. Biochem., 24, 1307-1314.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 AMP activation Bos taurus
2.7.1.11 D-fructose 1,6-bisphosphate activation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 86000
-
 1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis Bos taurus
2.7.1.11 88000
-
 1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis Bos taurus
2.7.1.11 92000
-
 1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis Bos taurus
2.7.1.11 400000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Bos taurus
2.7.1.11 Cibacron Blue F3GA affinity chromatography, ion-exchange, gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 parotid gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 67.5
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Bos taurus ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 tetramer 1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis Bos taurus