BRENDA - Enzyme Database

Characterization of dolichol kinase from soybean microsomes

Ravi, K.; Rip, J.W.; Carroll, K.K.; Biochim. Biophys. Acta 875, 618-625 (1986)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
Triton X-100
-
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
-
Glycine max
2.7.1.108
CTP
-
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.055
-
dolichol
pH 7.4, 37°C
Glycine max
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Glycine max
16020
-
2.7.1.108
microsome
-
Glycine max
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Mg2+
low level of stimulation noted, 80% of maximal activity is observed in absence of divalent kcations
Glycine max
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Glycine max
-
CDP + dolichyl phosphate
-
Glycine max
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Glycine max
-
soybean
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
seedling
-
Glycine max
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640398
Glycine max
CDP + dolichyl phosphate
-
640398
Glycine max
?
2.7.1.108
additional information
CTP or other phosphate group donors like ATP, GTP or UTP are not required for maximal activity
640398
Glycine max
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7
8
second optimum at pH 9.5
Glycine max
2.7.1.108
9.5
-
second optimum at pH 7.0-8.0
Glycine max
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
4
11
no second optimum with Tris-HCl, activity increases fairly linearly between pH 8.0 and 10.0, activity decreases about 25% between pH 9.5 and 10.0 in Chaps buffer
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
Triton X-100
-
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
-
Glycine max
2.7.1.108
CTP
-
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.055
-
dolichol
pH 7.4, 37°C
Glycine max
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Glycine max
16020
-
2.7.1.108
microsome
-
Glycine max
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Mg2+
low level of stimulation noted, 80% of maximal activity is observed in absence of divalent kcations
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Glycine max
-
CDP + dolichyl phosphate
-
Glycine max
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
seedling
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640398
Glycine max
CDP + dolichyl phosphate
-
640398
Glycine max
?
2.7.1.108
additional information
CTP or other phosphate group donors like ATP, GTP or UTP are not required for maximal activity
640398
Glycine max
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7
8
second optimum at pH 9.5
Glycine max
2.7.1.108
9.5
-
second optimum at pH 7.0-8.0
Glycine max
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
4
11
no second optimum with Tris-HCl, activity increases fairly linearly between pH 8.0 and 10.0, activity decreases about 25% between pH 9.5 and 10.0 in Chaps buffer
Glycine max