BRENDA - Enzyme Database

Subcellular localization and substrate specificity of dolichol kinase from rat liver

Keller, R.K.; Rottler, G.D.; Cafmeyer, N.; Adair, W.L.; Biochim. Biophys. Acta 719, 118-125 (1982)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
cytoplasm
-
Rattus norvegicus
5737
-
2.7.1.108
endoplasmic reticulum
-
Rattus norvegicus
5783
-
2.7.1.108
lysosome
-
Rattus norvegicus
5764
-
2.7.1.108
membrane
-
Rattus norvegicus
16020
-
2.7.1.108
microsome
-
Rattus norvegicus
-
-
2.7.1.108
mitochondrion
-
Rattus norvegicus
5739
-
2.7.1.108
nucleus
-
Rattus norvegicus
5634
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Rattus norvegicus
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
CDP + dolichyl phosphate
-
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
Rattus norvegicus Sprague-Dawley
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
CDP + dolichyl phosphate
-
Rattus norvegicus Sprague-Dawley
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Rattus norvegicus
-
rat; Sprague-Dawley
-
2.7.1.108
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.108
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.108
0.15
-
relative specific activity in the soluble fraction
Rattus norvegicus
2.7.1.108
0.21
-
relative specific activity in the mitochondrial lysosomal fraction
Rattus norvegicus
2.7.1.108
0.52
-
relative specific activity in the nuclear fraction
Rattus norvegicus
2.7.1.108
3.8
-
relative specific activity in the microsomal fraction, rough and smooth endoplasmic reticulum are found to have higher specific activities than the Golgi fraction
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
pig liver dolichol as substrate, R,S-dolichol-11, dolichol-16, dolichol-19
640393
Rattus norvegicus
CDP + dolichyl phosphate
-
640393
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
640393
Rattus norvegicus
CDP + dolichyl phosphate
-
640393
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
pig liver dolichol as substrate, R,S-dolichol-11, dolichol-16, dolichol-19
640393
Rattus norvegicus Sprague-Dawley
CDP + dolichyl phosphate
-
640393
Rattus norvegicus Sprague-Dawley
?
2.7.1.108
CTP + dolichol
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
640393
Rattus norvegicus Sprague-Dawley
CDP + dolichyl phosphate
-
640393
Rattus norvegicus Sprague-Dawley
?
2.7.1.108
CTP + ficaprenol
-
640393
Rattus norvegicus
CDP + ficaprenyl phosphate
-
-
-
?
2.7.1.108
CTP + ficaprenol
-
640393
Rattus norvegicus Sprague-Dawley
CDP + ficaprenyl phosphate
-
-
-
?
2.7.1.108
CTP + polyprenol
polyprenol-16, alpha-trans-polyprenol-16, polyprenol-19
640393
Rattus norvegicus
CDP + polyprenyl phosphate
-
-
-
?
2.7.1.108
CTP + polyprenol
polyprenol-16, alpha-trans-polyprenol-16, polyprenol-19
640393
Rattus norvegicus Sprague-Dawley
CDP + polyprenyl phosphate
-
-
-
?
2.7.1.108
additional information
microsomal enzyme shows a marked preference for saturation of the alpha-isoprene, dolichol-16 and dolichol-19 are 2.5fold more active than the corresponding polyprenols, the enzyme is twice as active against naturally occurring polyprenol-16 (alpha-cis-isoprene) compared to synthetic alpha-trans-polyprenol-16, all-trans-2,3-dihydrosolanesol and solanesol are not substrates
640393
Rattus norvegicus
?
-
-
-
-
2.7.1.108
additional information
microsomal enzyme shows a marked preference for saturation of the alpha-isoprene, dolichol-16 and dolichol-19 are 2.5fold more active than the corresponding polyprenols, the enzyme is twice as active against naturally occurring polyprenol-16 (alpha-cis-isoprene) compared to synthetic alpha-trans-polyprenol-16, all-trans-2,3-dihydrosolanesol and solanesol are not substrates
640393
Rattus norvegicus Sprague-Dawley
?
-
-
-
-
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
cytoplasm
-
Rattus norvegicus
5737
-
2.7.1.108
endoplasmic reticulum
-
Rattus norvegicus
5783
-
2.7.1.108
lysosome
-
Rattus norvegicus
5764
-
2.7.1.108
membrane
-
Rattus norvegicus
16020
-
2.7.1.108
microsome
-
Rattus norvegicus
-
-
2.7.1.108
mitochondrion
-
Rattus norvegicus
5739
-
2.7.1.108
nucleus
-
Rattus norvegicus
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Rattus norvegicus
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
CDP + dolichyl phosphate
-
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
Rattus norvegicus Sprague-Dawley
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
CDP + dolichyl phosphate
-
Rattus norvegicus Sprague-Dawley
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.108
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.108
0.15
-
relative specific activity in the soluble fraction
Rattus norvegicus
2.7.1.108
0.21
-
relative specific activity in the mitochondrial lysosomal fraction
Rattus norvegicus
2.7.1.108
0.52
-
relative specific activity in the nuclear fraction
Rattus norvegicus
2.7.1.108
3.8
-
relative specific activity in the microsomal fraction, rough and smooth endoplasmic reticulum are found to have higher specific activities than the Golgi fraction
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
pig liver dolichol as substrate, R,S-dolichol-11, dolichol-16, dolichol-19
640393
Rattus norvegicus
CDP + dolichyl phosphate
-
640393
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
640393
Rattus norvegicus
CDP + dolichyl phosphate
-
640393
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
pig liver dolichol as substrate, R,S-dolichol-11, dolichol-16, dolichol-19
640393
Rattus norvegicus Sprague-Dawley
CDP + dolichyl phosphate
-
640393
Rattus norvegicus Sprague-Dawley
?
2.7.1.108
CTP + dolichol
enzyme can function in augmenting the steady-state levels of dolichyl phosphate by phosphorylating preexisting dolichol molecules
640393
Rattus norvegicus Sprague-Dawley
CDP + dolichyl phosphate
-
640393
Rattus norvegicus Sprague-Dawley
?
2.7.1.108
CTP + ficaprenol
-
640393
Rattus norvegicus
CDP + ficaprenyl phosphate
-
-
-
?
2.7.1.108
CTP + ficaprenol
-
640393
Rattus norvegicus Sprague-Dawley
CDP + ficaprenyl phosphate
-
-
-
?
2.7.1.108
CTP + polyprenol
polyprenol-16, alpha-trans-polyprenol-16, polyprenol-19
640393
Rattus norvegicus
CDP + polyprenyl phosphate
-
-
-
?
2.7.1.108
CTP + polyprenol
polyprenol-16, alpha-trans-polyprenol-16, polyprenol-19
640393
Rattus norvegicus Sprague-Dawley
CDP + polyprenyl phosphate
-
-
-
?
2.7.1.108
additional information
microsomal enzyme shows a marked preference for saturation of the alpha-isoprene, dolichol-16 and dolichol-19 are 2.5fold more active than the corresponding polyprenols, the enzyme is twice as active against naturally occurring polyprenol-16 (alpha-cis-isoprene) compared to synthetic alpha-trans-polyprenol-16, all-trans-2,3-dihydrosolanesol and solanesol are not substrates
640393
Rattus norvegicus
?
-
-
-
-
2.7.1.108
additional information
microsomal enzyme shows a marked preference for saturation of the alpha-isoprene, dolichol-16 and dolichol-19 are 2.5fold more active than the corresponding polyprenols, the enzyme is twice as active against naturally occurring polyprenol-16 (alpha-cis-isoprene) compared to synthetic alpha-trans-polyprenol-16, all-trans-2,3-dihydrosolanesol and solanesol are not substrates
640393
Rattus norvegicus Sprague-Dawley
?
-
-
-
-