BRENDA - Enzyme Database

Properties of a dolichol phosphokinase activity associated with rat liver microsomes

Rip, J.W.; Carroll, K.K.; Can. J. Biochem. 58, 1051-1057 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
dithiothreitol
slight stimulation of activity
Rattus norvegicus
2.7.1.108
Triton X-100
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
-
Rattus norvegicus
2.7.1.108
EDTA
-
Rattus norvegicus
2.7.1.108
additional information
product of the reaction, dolichyl phosphate, is noninhibitory, no inhibition by dithiothreitol
Rattus norvegicus
2.7.1.108
Triton X-100
above 0.5% w/v, activation below
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.05
-
dolichol
pH 7.4, 37C
Rattus norvegicus
2.7.1.108
4
-
CTP
pH 7.4, 37C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Rattus norvegicus
2.7.1.108
Mg2+
-
Rattus norvegicus
2.7.1.108
Mn2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Rattus norvegicus
-
CDP + dolichyl phosphate
-
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
Rattus norvegicus Wistar
-
CDP + dolichyl phosphate
-
Rattus norvegicus Wistar
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Rattus norvegicus
-
rat; Wistar
-
2.7.1.108
Rattus norvegicus Wistar
-
Wistar
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640391
Rattus norvegicus
CDP + dolichyl phosphate
-
640391
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
-
640391
Rattus norvegicus Wistar
CDP + dolichyl phosphate
-
640391
Rattus norvegicus Wistar
?
2.7.1.108
additional information
ATP cannot replace CTP
640391
Rattus norvegicus
?
-
-
-
-
2.7.1.108
additional information
ATP cannot replace CTP
640391
Rattus norvegicus Wistar
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7
7.5
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
4
10
about half-maximal activity at pH 6.0 and 8.0
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
dithiothreitol
slight stimulation of activity
Rattus norvegicus
2.7.1.108
Triton X-100
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
-
Rattus norvegicus
2.7.1.108
EDTA
-
Rattus norvegicus
2.7.1.108
additional information
product of the reaction, dolichyl phosphate, is noninhibitory, no inhibition by dithiothreitol
Rattus norvegicus
2.7.1.108
Triton X-100
above 0.5% w/v, activation below
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.05
-
dolichol
pH 7.4, 37C
Rattus norvegicus
2.7.1.108
4
-
CTP
pH 7.4, 37C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Rattus norvegicus
2.7.1.108
Mg2+
-
Rattus norvegicus
2.7.1.108
Mn2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Rattus norvegicus
-
CDP + dolichyl phosphate
-
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
Rattus norvegicus Wistar
-
CDP + dolichyl phosphate
-
Rattus norvegicus Wistar
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640391
Rattus norvegicus
CDP + dolichyl phosphate
-
640391
Rattus norvegicus
?
2.7.1.108
CTP + dolichol
-
640391
Rattus norvegicus Wistar
CDP + dolichyl phosphate
-
640391
Rattus norvegicus Wistar
?
2.7.1.108
additional information
ATP cannot replace CTP
640391
Rattus norvegicus
?
-
-
-
-
2.7.1.108
additional information
ATP cannot replace CTP
640391
Rattus norvegicus Wistar
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7
7.5
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.108
4
10
about half-maximal activity at pH 6.0 and 8.0
Rattus norvegicus