BRENDA - Enzyme Database

Enzymatic phosphorylation of dolichol in central nervous tissue

Burton, W.A.; Scher, M.G.; Waechter, C.J.; J. Biol. Chem. 254, 7129-7136 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
2-mercaptoethanol
slight stimulation
Bos taurus
2.7.1.108
monothioglycerol
slight stimulation
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
end product inhibition
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.0065
-
CTP
pH 7.0, 37°C
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Bos taurus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Bos taurus
2.7.1.108
Mg2+
-
Bos taurus
2.7.1.108
Mn2+
-
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Bos taurus
newly formed dolichyl monophosphate, phosphorylated via CTP is available for biosynthesis of mannosylphosphoryldolichol and N-acetylglucosaminylpyrophosphoryldolichol
CDP + dolichyl phosphate
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.108
Bos taurus
-
calf
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
brain
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.108
-17°C, stable for at least 1 month
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640390
Bos taurus
CDP + dolichyl phosphate
-
640390
Bos taurus
?
2.7.1.108
CTP + dolichol
newly formed dolichyl monophosphate, phosphorylated via CTP is available for biosynthesis of mannosylphosphoryldolichol and N-acetylglucosaminylpyrophosphoryldolichol
640390
Bos taurus
CDP + dolichyl phosphate
-
640390
Bos taurus
?
2.7.1.108
additional information
ATP and GTP are not effective substrates compared to CTP
640390
Bos taurus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7
-
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.108
2-mercaptoethanol
slight stimulation
Bos taurus
2.7.1.108
monothioglycerol
slight stimulation
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.108
CDP
end product inhibition
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.108
0.0065
-
CTP
pH 7.0, 37°C
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.108
membrane
-
Bos taurus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.108
Ca2+
-
Bos taurus
2.7.1.108
Mg2+
-
Bos taurus
2.7.1.108
Mn2+
-
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.108
CTP + dolichol
Bos taurus
newly formed dolichyl monophosphate, phosphorylated via CTP is available for biosynthesis of mannosylphosphoryldolichol and N-acetylglucosaminylpyrophosphoryldolichol
CDP + dolichyl phosphate
-
Bos taurus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.108
brain
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.108
-17°C, stable for at least 1 month
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.108
CTP + dolichol
-
640390
Bos taurus
CDP + dolichyl phosphate
-
640390
Bos taurus
?
2.7.1.108
CTP + dolichol
newly formed dolichyl monophosphate, phosphorylated via CTP is available for biosynthesis of mannosylphosphoryldolichol and N-acetylglucosaminylpyrophosphoryldolichol
640390
Bos taurus
CDP + dolichyl phosphate
-
640390
Bos taurus
?
2.7.1.108
additional information
ATP and GTP are not effective substrates compared to CTP
640390
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.108
7
-
-
Bos taurus