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Literature summary extracted from

  • Lau, F.W.; Chen, X.; Bowie, J.U.
    Active sites of diacylglycerol kinase from Escherichia coli are shared between subunits (1999), Biochemistry, 38, 5521-5527.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 A14Q significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes, subunit mixing experiments of mutant enzymes Escherichia coli
2.7.1.107 D95N significantly impaired catalytic function, without evidence of gross structural alterations. Km-value for MgATP2- raises 18fold, subunit mixing experiments of mutant enzymes Escherichia coli
2.7.1.107 E69C mutant enzyme has an altered structure even in SDS Escherichia coli
2.7.1.107 E76L significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes Escherichia coli
2.7.1.107 K94L significantly impaired catalytic function, without evidence of gross structural alterations. Km-value for MgATP2- raises 13fold, subunit mixing experiments of mutant enzymes Escherichia coli
2.7.1.107 N72S significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 additional information
-
 additional information
-
 Escherichia coli
2.7.1.107 0.12
-
 ATP wild-type enzyme Escherichia coli
2.7.1.107 0.12
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.1.107 0.14
-
 ATP mutant enzyme E76L Escherichia coli
2.7.1.107 0.14
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.1.107 0.33
-
 ATP mutant enzyme E69C Escherichia coli
2.7.1.107 0.33
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.1.107 0.44
-
 ATP mutant enzyme N72S Escherichia coli
2.7.1.107 0.44
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.1.107 0.91
-
 ATP mutant enzyme A14Q Escherichia coli
2.7.1.107 0.91
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.1.107 1.5
-
 ATP mutant enzyme K94V Escherichia coli
2.7.1.107 1.5
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.1.107 2.1
-
 ATP mutant enzyme D95N Escherichia coli
2.7.1.107 2.1
-
 ATP ATP in form of MgATP2- Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane
-
 Escherichia coli 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
 Escherichia coli ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?