BRENDA - Enzyme Database

Diacylglycerol kinase from pig brain. Purification and phospholipid dependencies

Kanoh, H.; Kondoh, H.; Ono, T.; J. Biol. Chem. 258, 1767-1774 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
deoxycholate
enhances activity
Sus scrofa
2.7.1.107
lysophosphatidylcholine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
phosphatidylcholine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
phosphatidylethanolamine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
phosphatidylserine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
Phospholipid
enhances activity. Activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
sphingomyelin
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
phosphatidate
moderate
Sus scrofa
2.7.1.107
phosphatidylglycerol
inhibits phosphatidylcholine-dependent kinase activity
Sus scrofa
2.7.1.107
phosphatidylinositol
2.5 mol% results in 50% inhibition of the phosphatidylcholine-dependent kinase activity
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
0.3
-
ATP
pH 7.4, 30°C
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.107
76000
-
gel filtration
Sus scrofa
2.7.1.107
78000
-
1 * 78000, SDS-PAGE
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.107
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.107
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.107
8
-
-
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.107
4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week
Sus scrofa
2.7.1.107
4°C, purified enzyme, 50% loss of activity after 3 days
Sus scrofa
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.107
monomer
1 * 78000, SDS-PAGE
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
7.4
-
phosphatidylcholine-dependent activity
Sus scrofa
2.7.1.107
8
-
broad, deoxycholate-dependent or phosphatidylcholine-dependent activity
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.107
6.5
9
pH 6.5: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, phosphatidylcholine-dependent activity
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
deoxycholate
enhances activity
Sus scrofa
2.7.1.107
lysophosphatidylcholine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
phosphatidylcholine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
phosphatidylethanolamine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
phosphatidylserine
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
Phospholipid
enhances activity. Activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
2.7.1.107
sphingomyelin
activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
phosphatidate
moderate
Sus scrofa
2.7.1.107
phosphatidylglycerol
inhibits phosphatidylcholine-dependent kinase activity
Sus scrofa
2.7.1.107
phosphatidylinositol
2.5 mol% results in 50% inhibition of the phosphatidylcholine-dependent kinase activity
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
0.3
-
ATP
pH 7.4, 30°C
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.107
76000
-
gel filtration
Sus scrofa
2.7.1.107
78000
-
1 * 78000, SDS-PAGE
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.107
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.107
8
-
-
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.107
4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week
Sus scrofa
2.7.1.107
4°C, purified enzyme, 50% loss of activity after 3 days
Sus scrofa
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.107
monomer
1 * 78000, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
7.4
-
phosphatidylcholine-dependent activity
Sus scrofa
2.7.1.107
8
-
broad, deoxycholate-dependent or phosphatidylcholine-dependent activity
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.107
6.5
9
pH 6.5: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, phosphatidylcholine-dependent activity
Sus scrofa