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Literature summary extracted from

  • Kanoh, H.; Akesson, B.
    Properties of microsomal and soluble diacylglycerol kinase in rat liver (1978), Eur. J. Biochem., 85, 225-232.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.107 microsomal activity is more unstable on storage at 0°C than the soluble enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 GTP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 additional information
-
 additional information
-
 Rattus norvegicus
2.7.1.107 0.16
-
 ATP pH 7.0, 37°C, microsomal enzyme Rattus norvegicus
2.7.1.107 0.17
-
 ATP pH 7.0, 37°C, soluble enzyme Rattus norvegicus
2.7.1.107 0.42
-
 GTP pH 7.0, 37°C, soluble enzyme Rattus norvegicus
2.7.1.107 0.63
-
 GTP pH 7.0, 37°C, microsomal enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 cytosol
-
 Rattus norvegicus 5829
-
2.7.1.107 microsome
-
 Rattus norvegicus
-
-
2.7.1.107 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+ required, optimal activity of microsomal enzyme at 2 mM, optimal activity of soluble enzyme at 10 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.107 -20°C, in presence of 1 mM dithiothreitol, microsomal and soluble enzyme stable for about 1 week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + ceramide hardly utilized Rattus norvegicus ADP + ceramide 3-phosphate
-
 ?
2.7.1.107 GTP + 1,2-diacyl-sn-glycerol
-
 Rattus norvegicus GDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 additional information diacylglycerol emulsion Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 7
-
-
 Rattus norvegicus