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Literature summary extracted from

  • Yada, Y.; Ozeki, T.; Kanoh, H.; Nozawa, Y.
    Purification and characterization of cytosolic diacylglycerol kinases of human platelets (1990), J. Biol. Chem., 265, 19237-19243.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 deoxycholate enhances activity of enzyme form DGK-II and DGK-III, enzyme form DGK-I is not much affected Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 octyl glucoside enzyme form DGK-I, DGK-II and DGK-III Homo sapiens
2.7.1.107 phosphatidylcholine enzyme form DGK-I, DGK-II and DGK-III Homo sapiens
2.7.1.107 R59022 inhibits DGK-II and to a lesser extent DGK-III, but little affects DGK-I Homo sapiens
2.7.1.107 Triton N-101 enzyme form DGK-I, DGK-II and DGK-III Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.05
-
 diolein pH 7.4, 30°C, enzyme form DGK-I Homo sapiens
2.7.1.107 0.065
-
 diolein pH 7.4, 30°C, enzyme form DGK-II Homo sapiens
2.7.1.107 0.08
-
 diolein pH 7.4, 30°C, enzyme form DGK-III Homo sapiens
2.7.1.107 0.15
-
 ATP pH 7.4, 30°C, enzyme form DGK-I Homo sapiens
2.7.1.107 0.245
-
 ATP pH 7.4, 30°C, enzyme form DGK-II Homo sapiens
2.7.1.107 0.45
-
 ATP pH 7.4, 30°C, enzyme form DGK-III Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 cytosol isoenzyme DGK-I, DGK-II and DGK-II Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+ required, optimal activity at 2.5 mM for DGK-I, 5.0 mM for DGK-II and 10.0 mM for DGK-III Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 58000
-
 x * 58000, enzyme form DGK-III, SDS-PAGE Homo sapiens
2.7.1.107 75000
-
 enzyme form DGK-II, gel filtration Homo sapiens
2.7.1.107 152000
-
 x * 152000, enzyme form DGK-I, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 isoenzyme DGK-I and DGK-II, DGK-II only partially purified Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 platelet isoenzyme DGK-I, DGK-II and DGK-II Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 2.207
-
 enzyme form DGK-II Homo sapiens
2.7.1.107 4.74
-
 enzyme form DGK-I Homo sapiens
2.7.1.107 6.753
-
 enzyme form DGK-III Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 2,3-dioleoyl-sn-glycerol
-
 Homo sapiens ADP + 2,3-diacyl-sn-glycerol 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 ? x * 152000, enzyme form DGK-I, SDS-PAGE Homo sapiens
2.7.1.107 ? x * 58000, enzyme form DGK-III, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.107 42
-
 5 min, complete loss of activity of DGK-II and DGK-III, about 10% loss of activity of enzyme form DGK-I Homo sapiens