BRENDA - Enzyme Database

Lipid-dependent membrane enzymes. Purification to homogeneity and further characterization of diacylglycerol kinase from Escherichia coli

Russ, E.; Kaiser, U.; Sandermann, H.; Eur. J. Biochem. 171, 335-342 (1988)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
diacylglycerol 3-phosphate
the enzyme apoprotein is attributed to a novel feedback activation involving diacylglycerol 3-phosphate
Escherichia coli
2.7.1.107
lauryl maltoside
activates in presence of 11 mM Triton X-100
Escherichia coli
2.7.1.107
n-hexyl beta-D-glucoside
activates in presence of 11 mM Triton X-100
Escherichia coli
2.7.1.107
octyl beta-glucoside
activates in presence of 11 mM Triton X-100
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.107
14000
-
x * 14000 Da, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.107
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.107
3.767
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
1,2-dipalmitoyl-sn-glycerol + GTP
-
640360
Escherichia coli
GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.107
?
x * 14000 Da, SDS-PAGE
Escherichia coli
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.107
100
-
the membrane preparation shows a threefold stimulation by heating for 5 min followed by a gradual loss of activity, half-life: about 1 h. Butane-1-ol-dissolved enzyme has a half-life of about 45 min
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
diacylglycerol 3-phosphate
the enzyme apoprotein is attributed to a novel feedback activation involving diacylglycerol 3-phosphate
Escherichia coli
2.7.1.107
lauryl maltoside
activates in presence of 11 mM Triton X-100
Escherichia coli
2.7.1.107
n-hexyl beta-D-glucoside
activates in presence of 11 mM Triton X-100
Escherichia coli
2.7.1.107
octyl beta-glucoside
activates in presence of 11 mM Triton X-100
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.107
14000
-
x * 14000 Da, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.107
3.767
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
1,2-dipalmitoyl-sn-glycerol + GTP
-
640360
Escherichia coli
GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.107
?
x * 14000 Da, SDS-PAGE
Escherichia coli
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.107
100
-
the membrane preparation shows a threefold stimulation by heating for 5 min followed by a gradual loss of activity, half-life: about 1 h. Butane-1-ol-dissolved enzyme has a half-life of about 45 min
Escherichia coli