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Literature summary extracted from

  • Schaap, D.; de Widt, J.; van der Wal, J.; Vandekerckhove, J.; van Damme, J.; Gussow, D.; Ploegh, H.L.; van Blitterswijk, W.J.; van der Bend, R.L.
    Purification, cDNA-cloning and expression of human diacylglycerol kinase (1990), FEBS Lett., 275, 151-158.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 deoxycholate the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine. Diacylglycerol analogs containing an unsaturated fatty acid at the sn-2 position give optimal enzyme activity irrespective of the presence of deoxycholate Homo sapiens
2.7.1.107 phosphatidylcholine
-
 Escherichia coli
2.7.1.107 phosphatidylcholine the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine Homo sapiens
2.7.1.107 phosphatidylserine
-
 Escherichia coli
2.7.1.107 phosphatidylserine the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 subcloning into the expression vector pMT-2 and transfection in COS-7 cells results in a 6-7fold increase in diacylglycerol kinase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.45
-
 1,2-diolein pH 7.5, 30°C Homo sapiens
2.7.1.107 0.5
-
 ATP pH 7.5, 30°C, without deoxycholate Homo sapiens
2.7.1.107 1.6
-
 ATP pH 7.5, 30°C, with 1 mM deoxycholate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane
-
 Homo sapiens 16020
-
2.7.1.107 membrane bound to Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 86000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Escherichia coli
-
-
-
2.7.1.107 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 leukocyte
-
 Homo sapiens
-
2.7.1.107 T-lymphocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 6.913
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-dipalmitoyl-sn-glycerol 15% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-O-hexadecyl-2-oleoyl-sn-glycerol 45.4% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 1-O-hexadecyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-palmitoyl-2-oleoyl-sn-glycerol 96.5% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
 Homo sapiens ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide Homo sapiens ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 2,3-dioleoyl-sn-glycerol
-
 Homo sapiens ADP + 2,3-diacyl-sn-glycerol 1-phosphate
-
 ?
2.7.1.107 ATP + 2-monooleoyl-rac-glycerol 10.7% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol Homo sapiens ADP + 2-monooleoyl-rac-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + sn-1,2-dioleoylglycerol
-
 Escherichia coli ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?
2.7.1.107 ATP + sn-1,2-dioleoylglycerol
-
 Homo sapiens ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 monomer
-
 Escherichia coli
2.7.1.107 monomer 1 * 86000, SDS-PAGE Homo sapiens