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Literature summary extracted from

  • Wissing, J.B.; Wagner, K.G.
    Diacylglycerol kinase from suspension cultured plant cells (1992), Plant Physiol., 98, 1148-1153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 deoxycholate stimulation Catharanthus roseus
2.7.1.107 phosphatidylglycerol effective stimulation Catharanthus roseus
2.7.1.107 phosphatidylinositol effective stimulation Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 ADP
-
 Catharanthus roseus
2.7.1.107 ATP
-
 Catharanthus roseus
2.7.1.107 ATPgammaS
-
 Catharanthus roseus
2.7.1.107 CDP
-
 Catharanthus roseus
2.7.1.107 CTP
-
 Catharanthus roseus
2.7.1.107 GDP
-
 Catharanthus roseus
2.7.1.107 GDPbetaS
-
 Catharanthus roseus
2.7.1.107 GTP
-
 Catharanthus roseus
2.7.1.107 IDP
-
 Catharanthus roseus
2.7.1.107 ITP
-
 Catharanthus roseus
2.7.1.107 phosphate
-
 Catharanthus roseus
2.7.1.107 UDP
-
 Catharanthus roseus
2.7.1.107 UTP
-
 Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 additional information
-
 additional information in presence of Triton X-100, used for purification, a biphasic dependency upon diacylglycerol is observed and the apparent Michaelis constant values for diacylglycerol decreases with decreasing Triton concentration Catharanthus roseus
2.7.1.107 0.102
-
 ATP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.104
-
 GTP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.9
-
 dioleoylglycerol pH 7.5, 25°C Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 cytosol no activity in cytosol Catharanthus roseus 5829
-
2.7.1.107 membrane intrinsic membrane-protein Catharanthus roseus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Catharanthus roseus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + sn-1,2-dioleoylglycerol
-
 Catharanthus roseus ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?
2.7.1.107 GTP + dioleoylglycerol
-
 Catharanthus roseus GDP + dioleoylglycerol 3-phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.107 0.038
-
 IDP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.043
-
 UDP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.058
-
 GDP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.06
-
 CDP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.081
-
 GTP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.095
-
 ADP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.102
-
 ATP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.123
-
 GDPbetaS pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.26
-
 UTP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.48
-
 CTP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 0.51
-
 ITP pH 7.5, 25°C Catharanthus roseus
2.7.1.107 2.5
-
 phosphate pH 7.5, 25°C Catharanthus roseus