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Literature summary extracted from

  • Bertrand, L.; Alessi, D.R.; Deprez, J.; Deak, M.; Viaene, E.; Rider, M.H.; Hue, L.
    Heart 6-phosphofructo-2-kinase activation by insulin results from Ser-466 and Ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase B (1999), J. Biol. Chem., 274, 30927-30933.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.105 Insulin activation of transfected heart enzyme in human embryonic kidney 293 cells by inducing the phosphorylation of Ser466 and Ser483, activation is mediated by 3-phosphoinositide-dependent kinase-1, protein kinase B is in vivo not essential, activation is sensitive to LY294002 and wortmannin and insensitive to rapaycin and PD98059 Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105 wild-type enzyme, S466E and S466E/S483E mutants are expressed in Escherichia coli BL21(DE3)pLysE and S483E mutant is expressed in BL21(DE3)pLysS, human embryonic kidney 293 cells are transiently transfected with vectors expressing wild-type heart enzyme Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 S466E mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme in vitro Bos taurus
2.7.1.105 S466E/S483E the double mutant is not phosphorylated by protein kinase B, mutation decreases Km of beta-D-fructose 6-phosphate in vitro Bos taurus
2.7.1.105 S483E mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme and mutation decreases citrate inhibition in vitro Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information comparison of Km of wild-type and mutant enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 61000
-
 x * 61000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 wild-type and mutant enzyme Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 ? x * 61000, SDS-PAGE Bos taurus