Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Villadsen, D.; Nielsen, T.H.
    N-terminal truncation affects the kinetics and structure of fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase from Arabidopsis thaliana (2001), Biochem. J., 359, 591-597.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.105 pyruvate
-
 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105 expression in Saccharomyces cerevisiae Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 additional information the deletion of N-terminal 318 amino acids abolishes the Michaelis-Menten kinetics of enzyme, Km of beta-D-fructose 6-phosphate is increased, whereas Km of ATP is not significantly altered. When the first 66 amino acids are deleted, the activity ration between 6-phosphofructo-2-kinase and beta-D-fructose 2,6-bisphosphatase is halved. The deletion of 125, 179, 249 and 318 amino acids results in progressive further decreases in the activity ratio and the activity ratio is reduced 4fold when N-terminus is deleted completely. The full-length enzyme is eluted as a tetramer, whereas the truncated enzymes are eluted as monomers Arabidopsis thaliana
3.1.3.46 additional information N-terminal truncation mutants, behave as monomers, show a decrease in the kinase/phosphatase ration by 4-fold, role of N-terminus for subunit assembly and kinetic properties Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 ADP
-
 Arabidopsis thaliana
2.7.1.105 AMP not Arabidopsis thaliana
2.7.1.105 ATP not Arabidopsis thaliana
2.7.1.105 dihydroxyacetone phosphate
-
 Arabidopsis thaliana
2.7.1.105 diphosphate not phosphate Arabidopsis thaliana
2.7.1.105 glycerate 2-phosphate
-
 Arabidopsis thaliana
2.7.1.105 phosphoenolpyruvate
-
 Arabidopsis thaliana
2.7.1.105 sn-glycerol 3-phosphate
-
 Arabidopsis thaliana
3.1.3.46 6-phosphogluconate
-
 Arabidopsis thaliana
3.1.3.46 D-fructose-1,6-bisphosphate
-
 Arabidopsis thaliana
3.1.3.46 D-fructose-6-phosphate
-
 Arabidopsis thaliana
3.1.3.46 D-glucose-1,6-bisphosphate
-
 Arabidopsis thaliana
3.1.3.46 additional information not inhibitory: diphosphate Arabidopsis thaliana
3.1.3.46 phosphate
-
 Arabidopsis thaliana
3.1.3.46 pyruvate
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information comparison of Km of wild-type and mutant enzyme Arabidopsis thaliana
2.7.1.105 0.19
-
 ATP pH 6.0, 25°C Arabidopsis thaliana
2.7.1.105 0.5
-
 beta-D-fructose 6-phosphate pH 6.0, 25°C Arabidopsis thaliana
3.1.3.46 0.000028
-
 D-fructose-1,6-bisphosphate pH 6.0, 25°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 phosphate
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 83000
-
 4 * 83000 Arabidopsis thaliana
2.7.1.105 370000
-
 gel filtration Arabidopsis thaliana
3.1.3.46 83000
-
 4 * 83000, deduced from gene sequence Arabidopsis thaliana
3.1.3.46 370000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Arabidopsis thaliana
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Arabidopsis thaliana Q9MB58
-
-
3.1.3.46 Arabidopsis thaliana Q9MB58 bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, recombinant enzyme, expressed in yeast
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Arabidopsis thaliana ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Arabidopsis thaliana ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Arabidopsis thaliana ?
-
 ?
3.1.3.46 D-fructose 2,6-bisphosphate + H2O
-
 Arabidopsis thaliana D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 tetramer 4 * 83000 Arabidopsis thaliana
3.1.3.46 tetramer 4 * 83000, deduced from gene sequence Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 25
-
 assay at Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.105 4
-
 10 min, enzyme is stable, both in the absence and in the presence of phosphate Arabidopsis thaliana
2.7.1.105 25
-
 about 76% of enzyme activity remains after 10 min, both in the absence and in the presence of phosphate Arabidopsis thaliana
2.7.1.105 42
-
 10 min, activation of enzyme and deletion mutant decreases rapidly, but the presence of phosphate protectes the enzyme activities from inactivation and 75% of enzyme activity remains after 15 min Arabidopsis thaliana
3.1.3.46 42
-
 10 min, inactivation, phosphate protects Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 0.13
-
 phosphoenolpyruvate pH 6.0, 25°C Arabidopsis thaliana
2.7.1.105 0.35
-
 sn-glycerol 3-phosphate pH 6.0, 25°C Arabidopsis thaliana
2.7.1.105 0.45
-
 glycerate 2-phosphate pH 6.0, 25°C Arabidopsis thaliana
2.7.1.105 1.35
-
 dihydroxyacetone phosphate pH 6.0, 25°C Arabidopsis thaliana
3.1.3.46 0.08
-
 6-phosphogluconate
-
 Arabidopsis thaliana
3.1.3.46 0.08
-
 D-fructose-1,6-bisphosphate
-
 Arabidopsis thaliana
3.1.3.46 0.1
-
 D-fructose-6-phosphate
-
 Arabidopsis thaliana
3.1.3.46 0.5
-
 phosphate
-
 Arabidopsis thaliana
3.1.3.46 0.85
-
 D-glucose-1,6-bisphosphate
-
 Arabidopsis thaliana