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Literature summary extracted from

  • Tominaga, N.; Tsujikawa, T.; Minami, Y.; Wu, R.F.; Watanabe, F.; Sakakibara, R.; Uyeda, K.
    Effect of replacement of the amino and the carboxyl termini of rat testis fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase with those of the liver and heart isozymes (1997), Arch. Biochem. Biophys., 347, 275-281.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105
-
 Rattus norvegicus
2.7.1.105
-
 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 additional information mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme Rattus norvegicus
2.7.1.105 additional information mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 phosphoenolpyruvate
-
 Bos taurus
2.7.1.105 phosphoenolpyruvate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.023
-
 beta-D-fructose 6-phosphate pH 7.5, 25°C, heart Bos taurus
2.7.1.105 0.023
-
 beta-D-fructose 6-phosphate comparison of Km of wild-type, mutant and mutant phosphorylated enzyme Rattus norvegicus
2.7.1.105 0.023
-
 beta-D-fructose 6-phosphate comparison of Km of wild-type, mutant and mutant phosphorylated enzyme Bos taurus
2.7.1.105 0.025
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C, liver, comparison of Km of wild-type, mutant and mutant phosphorylated enzyme Rattus norvegicus
2.7.1.105 0.04
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C, wild-type enzyme, testis Rattus norvegicus
2.7.1.105 0.061
-
 ATP pH 7.5, 30°C, liver Rattus norvegicus
2.7.1.105 0.083
-
 ATP pH 7.5, 25°C, heart Bos taurus
2.7.1.105 0.1
-
 ATP
-
 Bos taurus
2.7.1.105 0.1
-
 ATP pH 7.5, 30°C, testis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Bos taurus
-
-
-
2.7.1.105 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 mutant enzyme Rattus norvegicus
2.7.1.105 mutant enzyme Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 heart
-
 Rattus norvegicus
-
2.7.1.105 heart
-
 Bos taurus
-
2.7.1.105 liver
-
 Rattus norvegicus
-
2.7.1.105 liver
-
 Bos taurus
-
2.7.1.105 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 25
-
 assay at Rattus norvegicus
2.7.1.105 25
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information comparison of kcat/Km values of wild-type and mutant enzymes Rattus norvegicus
2.7.1.105 additional information
-
 additional information comparison of kcat/Km values of wild-type and mutant enzymes Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 additional information
-
 additional information comparison of Ki of wild type and mutant enzymes Rattus norvegicus
2.7.1.105 0.014
-
 phosphoenolpyruvate pH 7.5, 30°C, testis Rattus norvegicus
2.7.1.105 0.08
-
 phosphoenolpyruvate pH 7.5, 30°C, liver Rattus norvegicus
2.7.1.105 0.1
-
 phosphoenolpyruvate pH 7.5, 30°C, heart Bos taurus