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Literature summary extracted from

  • Lange, A.J.; Li, L.; Vargas, A.M.; Pilkis, S.J.
    Expression of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli. Role of N-2 proline in degradation of the protein (1993), J. Biol. Chem., 268, 8078-8084.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105 mutant expressed in Escherichia coli BL21(DL3) Homo sapiens
2.7.1.105 mutant expressed in Escherichia coli BL21(DL3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 P2R kinetic properties of human liver mutant and rat wild-type enzyme are very similar Homo sapiens
2.7.1.105 P2R kinetic properties of human liver mutant and rat wild-type enzyme are very similar Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.019 0.021 beta-D-fructose 6-phosphate pH 7.8, in presence of phosphate Homo sapiens
2.7.1.105 0.019 0.021 beta-D-fructose 6-phosphate pH 7.8, in presence of phosphate Rattus norvegicus
2.7.1.105 0.12
-
 beta-D-fructose 6-phosphate pH 7.8 Rattus norvegicus
2.7.1.105 0.12 0.36 ATP pH 7.8 Rattus norvegicus
2.7.1.105 0.4
-
 ATP
-
 Rattus norvegicus
2.7.1.105 0.4
-
 ATP pH 7.8 Homo sapiens
2.7.1.105 1 1.1 beta-D-fructose 6-phosphate pH 7.8, absence of phosphate Homo sapiens
2.7.1.105 1 1.1 beta-D-fructose 6-phosphate pH 7.8, absence of phosphate Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 phosphate
-
 Homo sapiens
2.7.1.105 phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 additional information
-
 primary structures of human, rat and bovine liver enzyme Homo sapiens
2.7.1.105 additional information
-
 primary structures of human, rat and bovine liver enzyme Rattus norvegicus
2.7.1.105 55000
-
 2 * 55000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Homo sapiens
-
 human enzyme is 95% identical with rat or bovine enzyme
-
2.7.1.105 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 recombinant enzyme Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Homo sapiens
a
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 liver
-
 Homo sapiens
-
2.7.1.105 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.046
-
-
 Homo sapiens
2.7.1.105 0.046
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Homo sapiens ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer
-
 Homo sapiens
2.7.1.105 dimer 2 * 55000, SDS-PAGE Rattus norvegicus