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Literature summary extracted from

  • Ventura, F.; Rosa, J.L.; Ambrosio, S.; Pilkis, S.J.; Bartrons, R.
    Bovine brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Evidence for a neural-specific isozyme (1992), J. Biol. Chem., 267, 17939-17943.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate at physiological concentrations Bos taurus
2.7.1.105 phosphoenolpyruvate not Bos taurus
2.7.1.105 sn-glycerol 3-phosphate not Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.027
-
 beta-D-fructose 6-phosphate pH 7.1, 30°C Bos taurus
2.7.1.105 0.055
-
 ATP pH 7.1, 30°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 120000
-
 2 * 120000, SDS-PAGE Bos taurus
2.7.1.105 225000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein brain enzyme is phosphorylated, but not activated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 brain cortex Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.092
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer 2 * 120000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Bos taurus