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Literature summary extracted from

  • Kitamura, K.; Uyeda, K.
    Purification and characterization of myocardial fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase [published erratum appears in J Biol Chem 1989 Jan 25;264(3):1901] (1988), J. Biol. Chem., 263, 9027-9033.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.07
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C Bos taurus
2.7.1.105 0.26
-
 ATP
-
 Bos taurus
2.7.1.105 0.26
-
 ATP pH 7.5, 30°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 55000
-
 2 * 55000, SDS-PAGE Rattus norvegicus
2.7.1.105 118000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Bos taurus
-
-
-
2.7.1.105 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation site Bos taurus
2.7.1.105 phosphoprotein MW 58000 enzyme form Bos taurus
2.7.1.105 phosphoprotein phophorylation by cAMP-dependent protein kinase causes activation Rattus norvegicus
2.7.1.105 phosphoprotein phophorylation by cAMP-dependent protein kinase causes activation Bos taurus
2.7.1.105 phosphoprotein phosphorylation by protein kinase C causes activation Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 heart
-
 Rattus norvegicus
-
2.7.1.105 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.075
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer
-
 Bos taurus
2.7.1.105 dimer 2 * 55000, SDS-PAGE Rattus norvegicus
2.7.1.105 More
-
 Rattus norvegicus
2.7.1.105 More two isozymes: 58000 Da and 54000 Da, SDS-Page Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Rattus norvegicus
2.7.1.105 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 6
-
 of 54000 Da and 58000 Da isozymes Bos taurus