Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kountz, P.D.; El-Maghrabi, M.R.; Pilkis, S.J.
    Isolation and characterization of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from bovine liver (1985), Arch. Biochem. Biophys., 238, 531-543.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 sn-glycerol 3-phosphate i.e. alpha-glycerol phosphate Bos taurus
2.7.1.105 sn-glycerol 3-phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.0095 0.012 MgATP2- pH 7.5, 30°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ MgATP2 Rattus norvegicus
2.7.1.105 Mg2+ MgATP2 Bos taurus
2.7.1.105 phosphate
-
 Rattus norvegicus
2.7.1.105 phosphate
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 49000
-
 2 * 49000, SDS-PAGE Bos taurus
2.7.1.105 55000
-
 2 * 55000, SDS-PAGE Rattus norvegicus
2.7.1.105 102000
-
 gel filtration Bos taurus
2.7.1.105 110000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Bos taurus
-
-
-
2.7.1.105 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 liver
-
 Rattus norvegicus
-
2.7.1.105 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.042
-
-
 Bos taurus
2.7.1.105 0.06
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate via phosphorylenzyme intermediate r
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate via phosphorylenzyme intermediate r
2.7.1.105 MgATP2- + beta-D-fructose 6-phosphate
-
 Bos taurus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer 2 * 55000, SDS-PAGE Rattus norvegicus
2.7.1.105 dimer 2 * 49000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 8
-
 rat Rattus norvegicus
2.7.1.105 8.5
-
-
 Rattus norvegicus
2.7.1.105 8.5
-
 bovine Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.105 7.3 9.5 about half-maximal activity at pH 7.3 and 9.5 Bos taurus