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Literature summary extracted from

  • Pilkis, S.J.; Regen, D.M.; Stewart, H.B.; Pilkis, J.; Pate, T.M.; El-Maghrabi, M.R.
    Evidence for two catalytic sites on 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase. Dynamics of substrate exchange and phosphoryl enzyme formation (1984), J. Biol. Chem., 259, 949-958.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 ADP product inhibition Rattus norvegicus
2.7.1.105 AMP not Rattus norvegicus
2.7.1.105 beta-D-fructose 2,6-bisphosphate product inhibition Rattus norvegicus
2.7.1.105 citrate not Rattus norvegicus
2.7.1.105 sn-glycerol 3-phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information
-
 Rattus norvegicus
2.7.1.105 0.05
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C, with ATP, in presence of phosphate Rattus norvegicus
2.7.1.105 0.1
-
 ATP pH 7.5, 30°C Rattus norvegicus
2.7.1.105 0.5
-
 MgATP2- pH 7.5, 30°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ MgATP2 Rattus norvegicus
2.7.1.105 phosphate activation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate mechanism Rattus norvegicus
a
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate via phosphorylenzyme intermediate r
2.7.1.105 MgATP2- + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ?
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?
2.7.1.105 additional information isotope exchange reaction between ATP and ADP in the absence of fructose 6-phosphate Rattus norvegicus ?
-
 ?
2.7.1.105 additional information with discrete catalytic sites Rattus norvegicus ?
-
 ?
2.7.1.105 additional information isotope exchange between fructose 6-phosphate and fructose 2,6-bisphosphate in the absence of adenine nucleotides Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Rattus norvegicus