Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • El-Maghrabi, M.R.; Claus, T.H.; Pilkis, J.; Pilkis, S.J.
    Partial purification of a rat liver enzyme that catalyzes the formation of fructose 2,6-bisphosphate (1981), Biochem. Biophys. Res. Commun., 101, 1071-1077.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.2
-
 ATP pH 7.5, 22°C Rattus norvegicus
2.7.1.105 0.5
-
 MgATP2- pH 7.5, 30°C Rattus norvegicus
2.7.1.105 0.5
-
 beta-D-fructose 6-phosphate pH 7.5, 22°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.105 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ MgATP2 Rattus norvegicus
2.7.1.105 Mg2+ MgGTP2 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 85000 90000 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.0005
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate transfers gamma-phosphoryl group of ATP to hydroxyl group at C-2 of fructose 6-phosphate Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate no substrates are diphosphate, glucose 6-phosphate Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate not: ribose 5-phosphate Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 GTP + beta-D-fructose 6-phosphate less effective than ATP Rattus norvegicus GDP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 MgATP2- + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 22
-
 assay at Rattus norvegicus