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Literature summary extracted from

  • Ferro, A.J.; Marchitto, K.S.
    5-Methylthioribose kinase (Enterobacter aerogenes) (1983), Methods Enzymol., 94, 361-364.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.100 2-mercaptoethanol 20 mM, 70% of activity with dithiothreitol Klebsiella aerogenes
2.7.1.100 dithiothreitol required for activity, maximal activity at 20 mM Klebsiella aerogenes
2.7.1.100 dithiothreitol can partially be replaced by 20 mM 2-mercaptoethanol or glutathione Klebsiella aerogenes
2.7.1.100 glutathione 20 mM, 44% of activity with dithiothreitol Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.100 ADP
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.100 0.0081
-
 5-methylthioribose
-
 Klebsiella aerogenes
2.7.1.100 0.074
-
 ATP
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.100 Mg2+ maximal activity at 5 mM Klebsiella aerogenes
2.7.1.100 Mg2+ divalent metal ion is strictely required for activity, Mg2+ is most effective Klebsiella aerogenes
2.7.1.100 Mn2+ 20% of activity with Mg2+ Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose Klebsiella aerogenes may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine ADP + 5-methylthioribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.100 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.100 ammonium sulfate, Sephadex G-200, DEAE-cellulose Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.100 0.028
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.100 -20°C, 20% glycerol, at least 5 mg/ml, stable Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.100 ATP + 5-butylthioribose
-
 Klebsiella aerogenes ADP + 5-butylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-ethylthioribose
-
 Klebsiella aerogenes ADP + 5-ethylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-isobutylthioribose
-
 Klebsiella aerogenes ADP + 5-isobutylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-isopropylthioribose
-
 Klebsiella aerogenes ADP + 5-isopropylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-methylthioribose no activity with GTP and UTP Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-methylthioribose may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-propylthioribose
-
 Klebsiella aerogenes ADP + 5-propylthioribose 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.100 37
-
 assay at Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.100 7.3
-
-
 Klebsiella aerogenes