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Literature summary extracted from

  • Guranowski, A.
    Plant 5-methylthioribose kinase (1983), Plant Physiol., 71, 932-935.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.100 2-oxo-4-methylthiobutyrate 1 mM, 10% inhibition Lupinus luteus
2.7.1.100 5-Isobutylthioribose competitive vs. 1-phospho-5-methylthioribose Lupinus luteus
2.7.1.100 adenine 1 mM, 25% inhibition Lupinus luteus
2.7.1.100 ADP 1 mM, 66% inhibition Lupinus luteus
2.7.1.100 p-chloromercuribenzoate
-
 Lupinus luteus
2.7.1.100 S-adenosylhomocysteine 1 mM, 36% inhibition Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.100 0.0043
-
 5-methylthioribose pH 9.7, 35°C Lupinus luteus
2.7.1.100 0.0083
-
 ATP pH 9.7, 35°C Lupinus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.100 Mg2+ divalent metal ion is strictely required for activity, Mg2+ is most effective Lupinus luteus
2.7.1.100 Mn2+ divalent cation is strictley required for activity Lupinus luteus
2.7.1.100 Mn2+ 12% of activity with Mg2+ Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.100 70000
-
 gel filtration Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose Lupinus luteus enzyme may be involved in an alternative pathway of methionine synthesis in plant tissues ADP + 5-methylthioribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.100 Lupinus luteus
-
 yellow lupin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.100 ammonium sulfate, aminohexyl-Sepharose, Sephadex g-200, hydroxyapatite Bio-Gel Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.100 seed
-
 Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.100 0.1425
-
-
 Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.100 alphaATP + 5-methylthioribose
-
 Lupinus luteus alphaADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-isobutylthioribose
-
 Lupinus luteus ADP + 5-isobutylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-methylthioribose no activity with AMP, ADP and alpha,beta-CH2-ATP Lupinus luteus ADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 ATP + 5-methylthioribose enzyme may be involved in an alternative pathway of methionine synthesis in plant tissues Lupinus luteus ADP + 5-methylthioribose 1-phosphate
-
 ?
2.7.1.100 dATP + 5-methylthioribose 120% of activity with ATP Lupinus luteus dADP + 5-methylthioribose 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.100 35
-
 assay at Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.100 10 10.5
-
 Lupinus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.100 7 10.5 approx. 25% of maximal activity at pH 7.0 Lupinus luteus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.100 0.0014
-
 5-Isobutylthioribose
-
 Lupinus luteus