Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Petit, T.; Blazquez, M.A.; Gancedo, C.
    Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: Biochemical and molecular characterization of both hexokinases (1996), FEBS Lett., 378, 185-189.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.1 additional information hxk1+ expression increases strongly during growth in fructose or glycerol Schizosaccharomyces pombe
2.7.1.1 additional information hxk2+ expression is highest during growth in glycerol Schizosaccharomyces pombe

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 genes hxk1+ and hxk2+, encoding hexokinases 1 and 2, are cloned, sequenced and expressed in Saccharomyces cerevisiae WF8-2C Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 2-deoxy-D-glucose
-
 Schizosaccharomyces pombe
2.7.1.1 D-glucosamine
-
 Schizosaccharomyces pombe
2.7.1.1 additional information not inhibited by trehalose 6-phosphate; not inhibited by trehalose 6-phosphate Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.07
-
 D-mannose
-
 Schizosaccharomyces pombe
2.7.1.1 0.13
-
 D-mannose
-
 Schizosaccharomyces pombe
2.7.1.1 0.16
-
 D-glucose
-
 Schizosaccharomyces pombe
2.7.1.1 1.5
-
 D-fructose
-
 Schizosaccharomyces pombe
2.7.1.1 8.4 8.5 D-glucose
-
 Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 50000
-
 gel filtration Schizosaccharomyces pombe
2.7.1.1 53000
-
 gel filtration Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Schizosaccharomyces pombe P50521 hexokinase 2
-
2.7.1.1 Schizosaccharomyces pombe Q09756 hexokinase 1
-
2.7.1.1 Schizosaccharomyces pombe PB003 Q09756 hexokinase 1
-
2.7.1.1 Schizosaccharomyces pombe PB004 P50521 hexokinase 2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe PB003 ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe PB004 ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP 11% of the activity with fructose Schizosaccharomyces pombe ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP 59% of the activity with fructose Schizosaccharomyces pombe ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP 11% of the activity with fructose Schizosaccharomyces pombe PB003 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP 59% of the activity with fructose Schizosaccharomyces pombe PB004 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 42% of the activity with fructose Schizosaccharomyces pombe ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 23% of the activity with fructose Schizosaccharomyces pombe ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 23% of the activity with fructose Schizosaccharomyces pombe PB003 ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 42% of the activity with fructose Schizosaccharomyces pombe PB004 ADP + D-mannose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 50000, calculated from the amino acid sequence Schizosaccharomyces pombe
2.7.1.1 monomer 1 * 53000, calculated from the amino acid sequence Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Schizosaccharomyces pombe

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.5
-
 2-deoxy-D-glucose fructose as substrate Schizosaccharomyces pombe
2.7.1.1 2.1
-
 D-glucosamine fructose as substrate Schizosaccharomyces pombe
2.7.1.1 4
-
 2-deoxy-D-glucose fructose as substrate Schizosaccharomyces pombe
2.7.1.1 11
-
 D-glucosamine fructose as substrate Schizosaccharomyces pombe