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Literature summary extracted from

  • Cesar, M.deC.; Colepicolo, P.; Rosa, C.D.; Rosa, R.
    Purification and properties of buffalo (Bubalus bubalis) erythrocyte hexokinase (1997), Comp. Biochem. Physiol. B, 118, 395-401.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 2,3-diphosphoglycerate
-
 Bubalus bubalis
2.7.1.1 2-phosphoglycerate
-
 Bubalus bubalis
2.7.1.1 3-phosphoglycerate
-
 Bubalus bubalis
2.7.1.1 ADP mixed inhibitor versus both MgATP2- and glucose Bubalus bubalis
2.7.1.1 ATP uncomplexed, competitive to MgATP2-, erythrocyte enzyme Bubalus bubalis
2.7.1.1 D-fructose 1,6-diphosphate
-
 Bubalus bubalis
2.7.1.1 D-fructose 6-phosphate
-
 Bubalus bubalis
2.7.1.1 D-glucose 1,6-diphosphate
-
 Bubalus bubalis
2.7.1.1 D-glucose 6-phosphate strong inhibition, potential physiological effector Bubalus bubalis
2.7.1.1 Mg2+
-
 Bubalus bubalis
2.7.1.1 phosphate
-
 Bubalus bubalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.012
-
 D-glucose at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 0.024
-
 D-mannose at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 0.116
-
 D-glucosamine at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 0.125
-
 ATP MgATP2-, at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 0.134
-
 2-deoxy-D-glucose at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 1.44
-
 D-fructose at 30°C, pH 8.1 Bubalus bubalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 200000
-
 gel filtration Bubalus bubalis
2.7.1.1 200000
-
 tendency of 100 kDa hexokinases to aggregate Bubalus bubalis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Bubalus bubalis
-
 hexokinase I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 partial, 52240fold Bubalus bubalis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.1 ATP + D-hexose = ADP + D-hexose 6-phosphate random sequential mechanism Bubalus bubalis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 erythrocyte only one enzyme form Bubalus bubalis
-
2.7.1.1 erythrocyte hexokinase type I Bubalus bubalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 13.06
-
 at 30 °C, pH 8.1 Bubalus bubalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose 30% of the activity with glucose Bubalus bubalis ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP 46.3% of the activity with glucose Bubalus bubalis ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP 44.9% of the activity with glucose Bubalus bubalis ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP MgATP2- Bubalus bubalis ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 27.2% of the activity with glucose Bubalus bubalis ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 additional information not: UTP Bubalus bubalis ?
-
 ?
2.7.1.1 additional information not: N-acetyl-D-glucosamine Bubalus bubalis ?
-
 ?
2.7.1.1 additional information not: D-galactose Bubalus bubalis ?
-
 ?
2.7.1.1 additional information not: GTP Bubalus bubalis ?
-
 ?
2.7.1.1 additional information not: CTP Bubalus bubalis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 30
-
 assay at Bubalus bubalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8 8.6
-
 Bubalus bubalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Bubalus bubalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.034
-
 D-glucose 6-phosphate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 0.127
-
 D-glucose 1,6-diphosphate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 0.88
-
 D-fructose 6-phosphate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 1.5
-
 ADP versus MgATP2-, at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 3.6
-
 ATP at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 3.6
-
 ADP versus glucose, at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 4.6
-
 D-fructose 1,6-diphosphate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 14
-
 2,3-diphosphoglycerate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 14
-
 2-phosphoglycerate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 17.9
-
 phosphate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 21
-
 3-phosphoglycerate at 30°C, pH 8.1 Bubalus bubalis
2.7.1.1 36
-
 Mg2+ at 30°C, pH 8.1 Bubalus bubalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Bubalus bubalis isoelectric focusing 6.8 6.3