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Literature summary extracted from

  • Magnani, M.; Serafini, G.; Stocchi, V.; Bossu, M.; Dacha, M.
    Solubilization, purification, and properties of rabbit brain hexokinase (1982), Arch. Biochem. Biophys., 216, 449-454.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.1 D-glucose or D-fructose, 5 mM, stabilizes Oryctolagus cuniculus
2.7.1.1 glycerol, 9-15% v/v, stabilizes Oryctolagus cuniculus
2.7.1.1 sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.08
-
 D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 0.1
-
 D-mannose at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 0.4
-
 D-glucosamine at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 0.62
-
 ATP MgATP2-, at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 2
-
 2-deoxy-D-glucose at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 4.5
-
 D-fructose at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 10
-
 ITP MgITP2-, at 37°C, pH 8.1 Oryctolagus cuniculus
2.7.1.1 30
-
 UTP MgUTP2-, at 37°C, pH 8.1 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.1 mitochondrion
-
 Oryctolagus cuniculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 100000
-
 sucrose density gradient sedimentation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 717fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 brain
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 165
-
 at 37°C Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.1 4°C or -20°C, 5 mM sodium potassium phosphate buffer, pH 7.5, 9% v/v glycerol, 5 mM glucose, 3 mM mercaptoethanol, several days, stable Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose 54% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + 2-deoxy-D-glucose 84% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP 110% of the activity with glucose Oryctolagus cuniculus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP
-
 Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Oryctolagus cuniculus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP 82% of the activity with glucose Oryctolagus cuniculus ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose 12% of the activity with ATP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose MgITP Oryctolagus cuniculus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information not: N-acetyl-D-glucosamine Oryctolagus cuniculus ?
-
 ?
2.7.1.1 additional information not: D-galactose Oryctolagus cuniculus ?
-
 ?
2.7.1.1 additional information not: CTP Oryctolagus cuniculus ?
-
 ?
2.7.1.1 UTP + D-glucose 14% of the activity with ATP Oryctolagus cuniculus UDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 UTP + D-glucose MgUTP Oryctolagus cuniculus UDP + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 100000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8 8.5 in glycyl-glycine buffer Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Oryctolagus cuniculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Oryctolagus cuniculus isoelectric focusing 6.8 6.3